tailieunhanh - Phenotypic and genotypic characterization of enterococci from clinical isolates in a tertiary care hospital

Enterococci mainly being commensals in human faeces are now considered as an important cause of nosocomial infections. Of the infections, the commonly observed are urinary tract infections, abdominal infections followed by bacteremia, endocarditis and meningitis rarely. Speciation of Enterococcus aids in effective management of the infections caused by them. The initial treatment for enterococcal infections has become challenging due to development of resistance. The resistance has been observed mainly against aminoglycosides due to the presence of bi-functional gene. VRE strains are mostly isolated from patients with recurrent bacteremia, endovascular infections leading to increase deaths in the patients. To isolate and speciate enterococcal isolates from clinical samples, study the antibiotic susceptibility pattern and the genotype associated with the aminoglycoside and vancomycin resistance. Enterococcus isolates obtained from various sections were characterized by conventional phenotypic methods. The antibiotic susceptibility was studied by disc diffusion method. The resistant strains were further confirmed by MIC using automated methods following which the genotypic analysis was done. |