tailieunhanh - Process optimization for the production of β-Galactosidase using potential Lactobacillus cultures

Ten Lactobacillus cultures were considered for the study. Out of ten Lactobacillus cultures, V3 produced maximum β-galactosidase activity ( ), followed by NK2 and NK9 up to 24h than other cultures during β-galactosidase activity. Extraction of βgalactosidase was carried out by disruption of Lactobacillus cells using sonication at 3, 4 and 5 minute (pulse 30 second on / 10 seconds off and 35 to 55 % amplitudes), lysozymeEDTA (49 mg of lysozyme in ml of TE buffer) treatment, toluene acetone (9:1 [toluene: acetone]) treatment, sodium dodecyl sulphate (SDS) - chloroform (250 µl chloroform and 100 ml of % SDS) treatment. Out of 4 different treatments [sonication, lysozyme-EDTA, toluene-acetone and sodium dodecyl sulphate (SDS)-chloroform treatment] for β-galactosidase production, sonication treatment [V3 (), NK2 () and NK9 ()] was found to be best for production of β-galactosidase with optimized sonication period [5 min (pulse 15 sec off / 30 sec on at 55% amplitudes)]. |

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