tailieunhanh - Cytotoxic steroids found in Vietnamese lingzhi Ganoderma neo-japonicum

Recently, we collected fruit bodies of Ganoderma neo-japonicum in Lamdong Province and found that the EtOAc crude extract obtained, having an IC50 value of µg/mL, shows strong cytotoxicity against the KB cell line. This paper describes the isolation and cytotoxicity of four compounds from this mushroom. | JOURNAL OF SCIENCE OF HNUE Chemical and Biological Sci. 2014 Vol. 59 No. 9 pp. 25-29 This paper is available online at http CYTOTOXIC STEROIDS FOUND IN VIETNAMESE LINGZHI Ganoderma neo-japonicum Bui Thi Thu Hien1 Nguyen Trang Thu1 Nguyen Quang Tuyen1 Le Xuan Tham2 and Dang Ngoc Quang1 1 Faculty of Chemistry Hanoi National University of Education 2 Department of Science and Technology Lam Dong Abstract. A chemical investigation of the ethylacetate extract of fruit bodies of Vietnamese lingzhi Ganoderma neo-japonicum collected in Cat Tien National Park Lamdong Province has resulted in the isolation and structural elucidation of four fungal metabolites ergosta-4 6 8 14 22-tetraen-3-one 1 ganodermadiol 2 ergosterol 3 and ergosta-7 22-dien-3β-ol 4 through the use of various chromatographic and spectroscopic methods including ESI-MS and 2D NMR. In addition compounds 1 3 and 4 showed moderate cytotoxic activity toward KB cell lines with their IC50 values of and µg mL respectively. Keywords Ganoderma neo-japonicum cytotoxicity lingzhi steroid. 1. Introduction It is estimated that 60 species of the Ganodermataceae family can be found in Vietnam however only half of them have actually been found 9 . They have long been used in traditional Chinese Japanese Korean and Vietnamese medicine to treat a variety of conditions 3 . Our previous studies on the chemical constituents of Vietnamese lingzhi revealed that they contained various fungal metabolites such as cattienoids A-C and schisanlactone A from Tomophagus cattienensis 2 sterols from Amauroderma subresinosum 5 and lanostane-type triterpenoids from Ganoderma tropicum 6 . Recently we collected fruit bodies of Ganoderma neo-japonicum in Lamdong Province and found that the EtOAc crude extract obtained having an IC50 value of µg mL shows strong cytotoxicity against the KB cell line. This paper describes the isolation and cytotoxicity of four compounds from this mushroom. Received August