tailieunhanh - Use of RAPD marker for the assessment of genetic diversity of sesame (Sesamum indicum L.) varieties

Genetic diversity among 28 sesame (Sesamum indicum L.) varieties was examined at DNA level by means of random amplified polymorphic DNA (RAPD) analysis. Fifteen primers used produced a total of 132 RAPD fragments. Each primer generated 3 to 26 amplified fragments with an average of bands per primer. Based on pair-wise comparisons of RAPD amplification products, Nei and Li’s similarity coefficients were computed to assess the associations among the varieties. Pair-wise similarity indices varied from to . A UPGMA cluster analysis based on these genetic similarities located most of the varieties far apart from one another, showing a high level of polymorphism. Genetically, all the genotypes were classified into two major groups at similarity coefficient and these major groups are further divided into small clusters as the similarity coefficient level increases. | 2017 6 5 2523-2530 International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 6 Number 5 2017 pp. 2523-2530 Journal homepage http Original Research Article https Use of RAPD Marker for the Assessment of Genetic Diversity of Sesame Sesamum indicum L. Varieties Kanak Saxena and Rajani Bisen Department of Genetics and Plant Breeding College of Agriculture Jawaharlal Nehru Krishi Vishwa Vidyalaya Jabalpur . India Corresponding author ABSTRACT Genetic diversity among 28 sesame Sesamum indicum L. varieties was examined at DNA level by means of random amplified polymorphic DNA RAPD analysis. Fifteen primers Keywords used produced a total of 132 RAPD fragments. Each primer generated 3 to 26 amplified fragments with an average of bands per primer. Based on pair-wise comparisons of RAPD RAPD amplification products Nei and Li s similarity coefficients were computed to Genetic assess the associations among the varieties. Pair-wise similarity indices varied from to diversity . A UPGMA cluster analysis based on these genetic similarities located most of the Polymorphism varieties far apart from one another showing a high level of polymorphism. Genetically all the genotypes were classified into two major groups at similarity coefficient and Article Info these major groups are further divided into small clusters as the similarity coefficient level increases. Accession Swetha Til and AKT-101 was show maximum distance from each Accepted other. In conclusion even with the use of a limited set of primers RAPD technique 25 April 2017 revealed a high level of genetic variation among sesame varieties. This high level of Available Online genetic diversity among the genotypes suggested that RAPD technique is valuable for 10 May 2017 sesame systematic and can be helpful for competent choice of parents in breeding programs. Introduction Sesame Sesamum indicum .

• Nông nghiệp
• Genetic diversity
• RAPD marker for the assessment
• Genetic diversity of sesame
• Sesame varieties
• Similarity coefficient level increases
• Genetic variability
• Similarity coefficient
• Soybean genotypes
• Genetic diversity analysis
• Molecular marker based genetic diversity
• Molecular marker
• Maize genetic diversity
• Very easy digestible
• Abiotic factor
• Genetic diversity in sunflower
• Sunflower (Helianthus annuus L )
• Inter varietal hybridization programme
• Assessment of genetic diversity
• Genetic diversity among fenugreek
• Fenugreek genotypes
• ISSR markers
• Genetic improvement
• Sequence related amplified polymorphism
• Simple sequence repeat
• Genetic diversity of watermelon
• Brassica compestris
• Genetic diversity analysis based
• Molecular level
• Onion genotypes through RAPD
• Genetic diversity remarkably
• Genetic diversity in Gangatiri cow
• Gangatiri cow
• Employing RAPD marker
• Threatened cycads (cycadaceae)
• Conservation of genetic diversity
• High propagules
• Levels of genetic diversity
• Inter Intra cluster distance
• Chickpea genotypes
• Genetic diversity in reference set
• Genetic advance
• Rainfed rice
• Rainfed rice genotypes
• Genetic diversity in rainfed rice genotypes
• Bottle gourd
• Genetic diversity in bottle gourd
• Yield improvement
• Diversity analysis
• Genetic diversity analysis in sunflower
• Parental lines
• RAPD markers
• Solanum lycopersicon L
• Genetic diversity studies
• Genetic diversity studies in tomato
• D2 statistics
• Genetic divergence
• Random amplified polymorphic DNA
• Polymerase chain reaction
• Genetic diversity analysis of citrus cultivars
• D2 analysis
• Landraces and salinity
• Saline stress condition
• Genetic diversity in traditional landraces
• Rice for yield
• Principal component analysis
• Principal component analysis approach
• Brinjal (Solanum melongena L
• Pinus kwangtungensis
• Pa Co pine (Pinus kwangtungensis Chun ex Tsiang)
• RAPD and ISSR markers
• Genetic diversity in Pa Co pine
• Thirty five genotypes
• Oilseed brassica species
• Gossypium hirsutum L
• Cotton germplasm
• Agro morphological characterization
• PIC and marker
• Genetic diversity in hybrids of tomato
• Tomato (Solanum lycopersicum L
• SSR markers
• Molecular characterization
• Soybean varieties
• Genetic diversity assessment
• Agarose gel electrophoresis
• ISSR primers
• Susceptible genotypes
• Genetic diversity in upland cotton
• Molecular markers
• Mungbean [Vigna radiata (L ) Wilczek]
• Screening and identification
• Identification of ssr markers
• Genetic diversity for mungbean
• Mahalanobis D2 statistics
• Genetic diversity in okra
• West Godavari district during kharif season
• Twenty nine hybrids