tailieunhanh - Pseudomonas aeruginosa KPSE 3 – An endophytic PGPR for Bio control of potato phytopathogens

In the present study, an attempt has been made to isolate endophytic bacteria mainly belonging to Pseudomonas genus from potato possessing ability to work as plant growth promoting bacteria (PGPB) as well as biocontrol agent. | 2017 6 5 2954-2964 International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 6 Number 5 2017 pp. 2954-2964 Journal homepage http Original Research Article https Pseudomonas aeruginosa KPSE 3 An Endophytic PGPR for Bio Control of Potato Phytopathogens . Pandya and . Shelat Department of Microbiology B. A. College of Agriculture Anand Agricultural University Anand Gujarat India Corresponding author ABSTRACT Endophytic bacteria residing within the living plant tissue without causing harm to it have promise to be practically used in agriculture and included as active components of Integrated Disease Management IDM . In present study 4 strains of pseudomonad were isolated from surface sterilized plant parts viz. leaf root tuber and stem of potato Solanum tuberosum L. cv. Kufri Badshah by using King s B agar and preliminary characterized as Pseudomonas on the basis of Keywords morphological and biochemical characteristics. Among all the isolates KPSE3 was Endophytic emerged as best PGP strain showing production of siderophore and IAA Siderophore μg ml . Moreover KPSE3 has capacity of nitrogen fixation mg N g Phytopathogen glucose consumed and phosphate solubilization P μg ml at 3 DAI . The Eco-friendly. isolate KPSE3 also have broad spectrum antifungal activity with 15 growth Article Info inhibition of Pythium sp. 34 of Fusarium sp. and 40 of Macrophomina sp. In pot trial conditions potato tuber bacterization with KPSE3 in presence of Accepted phytopathogens viz. Fusarium sp. Macrophomina sp. and pythium sp. 26 April 2017 Available Online significantly influenced growth of Potato cv. Kufri Badshah. The endophytic 10 May 2017 isolate KPSE3 exhibiting the best PGP activity was further characterized at molecular level and identified as Pseudomonas aeruginosa Gene Bank Accessions Number- KC787580 which can be used for developing a cost - .