tailieunhanh - Evaluation of organic acid producing aspergillus niger isolates for the management of fusarium wilt of chickpea

The soil-borne necrotrophic fungal pathogen Fusarium oxysporum f. sp. Ciceris (FOC)infects chickpeaand causes wilt in any stage of plant from seedling to podding. Owing to climate change and ever changing nature of the pathogen, no resistant host cultivar is sustaining long against this disease. Hence, root resident Aspergillus niger isolates as native mycoflora were evaluated as bioagent against the FOC because chemical control has long been discouraged due to its circulation in food chain. In this study, we established the biocontrol potential of organic acid producing A. niger isolates under invitro and in-vivo conditions. | 2017 6 5 256-265 International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 6 Number 5 2017 pp. 256-265 Journal homepage http Original Research Article https Evaluation of Organic Acid Producing Aspergillus niger Isolates for the Management of Fusarium Wilt of Chickpea Swati Nayak and Vibha Department of Plant Pathology Jawaharlal Nehru Krishi Vishwa Vidyalaya Jabalpur 482 004 Madhya Pradesh India Corresponding author ABSTRACT The soil-borne necrotrophic fungal pathogen Fusarium oxysporum f. sp. Ciceris FOC infects chickpeaand causes wilt in any stage of plant from seedling to podding. Keywords Owing to climate change and ever changing nature of the pathogen no resistant host cultivar is sustaining long against this disease. Hence root resident Aspergillus niger Fungal isolates Fusarium wilt isolates as native mycoflora were evaluated as bioagent against the FOC because chemical Organic acids control has long been discouraged due to its circulation in food chain. In this study we Physiological established the biocontrol potential of organic acid producing A. niger isolates under in- parameters and vitro and in-vivo conditions. All the isolates produced tryptophan one proline three Disease incidence. histidine and nine hyproline and valine. The isolate1 was highly inhibitory towards the FOC under poison food technique while isolate9 was highly suppressive towards the Article Info pathogen under dual culture method. The maximum wilt reduction was recorded with Accepted isolate3 treatment that also helped the plant to retain maximum relative water content in 04 April 2017 leaves besides maintaining higher chlorophyll content. From the results it can be Available Online concluded that the response of bioagents under in-vitro conditions can vary under in-vivo 10 May 2017 conditions depending upon the inherent metabolite producing ability of the bioagent. This