tailieunhanh - RAPD analysis for genetic diversity and verification of hybridity in cowpea [Vigna unguiculata (L.) Walp.]

This study was thus undertaken to assess genetic diversity among the different parents used for hybridization and confirmation of hybrids of cowpea (Vigna unguiculata) by using randomly amplified polymorphic DNA (RAPD) markers. RAPD profiles for 11 genotypes were generated with 20 random decamer primers. Out of 20 primers screened 15 primers gave scorable DNA fragments and each of the 15 primers revealed various levels of polymorphism. These primers generated 476 DNA fragments in the average range of 167bp to 3300bp, of which 367 were polymorphic. |

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