tailieunhanh - Phenotypic virulence characterization of dichelobacter nodosus from the cases of ovine footrot in Andhra Pradesh and Telangana states of India

The aim of the study is to characterize isolates with respect to virulence. In the present investigation gelatin test and elastase test were used to study the virulence of isolates recovered in the study. | 2020 9 6 2924-2936 International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 9 Number 6 2020 Journal homepage http Original Research Article https Phenotypic Virulence Characterization of Dichelobacter nodosus from the Cases of Ovine Footrot in Andhra Pradesh and Telangana states of India S. Vijayalakshmi1 D. Sreenivasulu2 D. Raniprameela3 N. Vinodkumar4 and A. Karthik5 1 LRS Palamaneru SVVU India 2 SVVU Tirupati India 3 SLDL SVVU Tirupati India 4 Department of Microbiology CVSc Tirupati India 5 SRF SLDL Tirupati India Corresponding author ABSTRACT Footrot is a contagious disease affecting the interdigital epidermis and living Keywords tissues of digits of sheep and goats. The disease is being reported in Andhra Pradesh AP and Telangana states of India regularly causing significant economic Interdigital loss to the sheep husbandry. The causative agent is the anaerobic gram negative epidermis bacteria Dichelobacter nodosus. A total of sixteen isolates of have Economic loss RNA gene been recovered from footrot positive clinical samples collected from AP and Telangana state and further confirmed by PCR targeting 16Sr RNA gene. The Article Info isolates were further processed for virulence characterization by Gelatin gel test Accepted and elastase test. Gelatin gel test revealed of isolates as virulent of 21 May 2020 isolates as Intermediate and of isolates as benign. Whereas elastase test Available Online identified only of isolates as virulent of isolates as intermediate 10 June 2020 of isolates as benign the rest of the isolates did not exhibit any elastase activity. Introduction establish knowledge of virulence of the and the corresponding Footrot is highly contagious disease of sheep manifestations of footrot. were and goats caused by an obligatory anaerobic categorized as virulent .