tailieunhanh - Cloning and identification of an ACO gene involved in ethylene production pathway from Ginkgo biloba
1-Aminocyclopropane-1-carboxylic acid (ACC) oxidase (ACO), is one of the core enzymesin the ethylene biosynthesis pathway, which catalyzes the final step in ethylene biosynthesis converting ACC to ethylene, cyanide, CO2, dehydroascorbate and water with inputs of Fe(II), ascorbic, bicarbonate and the present study, a 1288 bp cDNA sequence fragment of the ACO gene (designated as GbACO) was isolated from ginkgo (Ginkgo biloba) leaves by using reverse transcriptase-polymerase chain reaction (RT-PCR) with two PCR primers designed according to the ginkgo transcriptome data. |
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