tailieunhanh - Comparative study on serodiagnosis of bovine leptospirosis by Microagglutination Test (MAT) and indirect ELISA

The present study was undertaken to identify the presence of anti-leptospiral antibodies in the bovine serum samples using two serological tests, a genus specific indirect ELISA and a serovar specific microagglutination test (MAT). A total of 250 serum samples from bovine leptospirosis suspected cases were sampled in and around Pondicherry (Southern India). The anit-leptospiral antibodies were detected by indirect ELISA using outer membrane proteins (OMP’s) of Leptospira interrogans serovar pomona as antigen and compared with MAT using six infecting serovars (hardjo, icterohaemorrhagiae, grippotyphosa, pomona, canicola and australis) as antigen. Statistical analysis of the data was performed using chi-square test. Out of 250 sera samples, 91 ( %) and 62 () were positive by indirect ELISA and MAT respectively. The indirect ELISA employed in this study revealed a very high sensitivity of % in comparison to % in MAT. Chi –square analysis revealed a significant difference (. P | Comparative study on serodiagnosis of bovine leptospirosis by Microagglutination Test MAT and indirect ELISA

• Nông nghiệp
• Bovine leptospirosis
• Indirect ELISA
• Outer membrane protein
• Microagglutination test
• Serodiagnosis of bovine leptospirosis
• Microscopic agglutination test
• Special reference
• Circulating antibodies against leptospira