tailieunhanh - A rapid method for titration of recombinant baculovirus stock based on bicistronic expression of green fluorescent protein (GFP) reporter
To determine the titer of baculovirus stock accurately based on green fluorescent protein (GFP) reporter, a recombinant baculovirus encoding FLAG-tagged protein and GFP reporter as bicistronic messenger was constructed. The bicistronic messenger RNA was transcribed using polyhedrin promoter and translated in infected Sf21 insect cells. The GFP reporter gene was expressed incap independent manner utilizing internal ribosome entry site (IRES) which helped in titration of baculovirus using end point titration method. The result of this study indicated that recombinant baculovirus expressing GFP reporter as bicistronic messenger facilitated in titration of baculovirus in stock in rapid and accurate manner. | A rapid method for titration of recombinant baculovirus stock based on bicistronic expression of green fluorescent protein GFP reporter
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