tailieunhanh - Characterization of Bt transgenic plants: A review
Insecticidal δ-endotoxins of Bacillus thuringiensis have acquired great significance in recent years because of their specificity to target insects and toxicity at very low concentrations. Genes from B. thuringiensis have been the most successful group of genes identified for use in genetic transformation of crops for pest control on a commercial scale. Successes of plant transformation include most of major economic and vegetable crops. Polymerase chain reaction (PCR) is commonly used technique for the presence of transgene in the putative transformants by DNA amplification. The Southern blot technique is used to confirm gene integration and copy numbers and Northern blot for gene expression in transgenic plants. Quantification Bt protein expressed in plants is performed by Enzyme Linked Immunosorbent Assay (ELISA). Bt transgenic plants were assessed for insect resistance against target pest/s by conducting insect bio-assays. | Characterization of Bt transgenic plants A review
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