tailieunhanh - Assessment of different methods for sap transmission of chilli leaf curl virus in Capsicum annuum L. and its validation through PCR amplification of AV1 coat protein gene

Chilli (Capsicum annuum L.) is an important spice crop severely affected by the virus causes leaf curl symptoms. Efficient transmission of Chilli Leaf Curl Virus was done by using a standardized buffer for sap inoculation to chilli plants. In present investigation, we have used three simple methods . inoculation by syringe, rubbing and by immersion (dip) for sap inoculation and found reproducible when the sap from the infected plants was inoculated onto healthy plants. Out of the three methods used, syringe inoculation was found most efficient method for sap transmission. Syringe inoculation caused most severe symptoms of leaf curl followed by immersion dip after 6 weeks after the inoculation. The sap transmission of chilli leaf curl virus was confirmed by coat protein gene amplification. PCR amplification showed the presence of 320bp single DNA fragment of AV1 coat protein gene that confirmed the presence of Chilli leaf curl virus in the sap transmitted plants. | Assessment of different methods for sap transmission of chilli leaf curl virus in Capsicum annuum L. and its validation through PCR amplification of AV1 coat protein gene

• Sinh học
• Sap transmission
• Gemini virus
• Coat protein
• AV1 coat protein gene
• Chilli leaf curl virus
• Sunflower necrosis virus
• Tobacco streak virus
• Sap inoculation
• Detection of sunflower necrosis virus
• Sunflower necrosis virus in sunflower
• Glycine max
• Host range
• Soybean mosaic virus
• Soybean mosaic virus in soybean
• Host range studies
• Exhibit any symptoms
• Dark green colour of leaves
• BMC Genomics
• Asia II 1
• Bemisia tabaci
• Phloem sap sucking pests
• Cryptic species
• Tomato yellow leaf curl virus
• Integrated approach
• Chilli leaf curl virus disease
• Coastal zone of odisha
• Leaf curl virus
• Per cent disease incidence
• Sustainable disease management practices
• Cropping system
• Host resistance
• Leaf pubescence
• Chilli (Capsicum frutescens L )
• Host plant resistance to leaf curl
• Murda complex
• Leaf curl
• Byadgi dabbi
• Murda complex disease in chilli
• Virus associated