tailieunhanh - Assessment of different methods for sap transmission of chilli leaf curl virus in Capsicum annuum L. and its validation through PCR amplification of AV1 coat protein gene

Chilli (Capsicum annuum L.) is an important spice crop severely affected by the virus causes leaf curl symptoms. Efficient transmission of Chilli Leaf Curl Virus was done by using a standardized buffer for sap inoculation to chilli plants. In present investigation, we have used three simple methods . inoculation by syringe, rubbing and by immersion (dip) for sap inoculation and found reproducible when the sap from the infected plants was inoculated onto healthy plants. Out of the three methods used, syringe inoculation was found most efficient method for sap transmission. Syringe inoculation caused most severe symptoms of leaf curl followed by immersion dip after 6 weeks after the inoculation. The sap transmission of chilli leaf curl virus was confirmed by coat protein gene amplification. PCR amplification showed the presence of 320bp single DNA fragment of AV1 coat protein gene that confirmed the presence of Chilli leaf curl virus in the sap transmitted plants. | Assessment of different methods for sap transmission of chilli leaf curl virus in Capsicum annuum L. and its validation through PCR amplification of AV1 coat protein gene