tailieunhanh - Sàng lọc vi khuẩn phân lập từ rừng ngập mặn có khả năng kháng khuẩn

Bài viết tiến hành phân lập vi khuẩn rừng ngập mặn, tách chiết và thử nghiệm khả năng kháng khuẩn, chủng tiềm năng được định danh bằng 16S rRNA. | Sàng lọc vi khuẩn phân lập từ rừng ngập mặn có khả năng kháng khuẩn Vietnam Journal of Marine Science and Technology Vol. 19 No. 4 2019 601 610 DOI https 1859-3097 19 4 12898 http jmst Screening of mangrove - derived bacteria from Ninh Ich Ninh Hoa Khanh Hoa for antimicrobial producers Pham Thi Mien Nguyen Van Khoa Institute of Oceanography VAST Vietnam E-mail Received 24 December 2018 Accepted 30 June 2019 2019 Vietnam Academy of Science and Technology VAST Abstract In the context of global climate changes mangroves are not only considered as an important ecological region but also as a source of exploitation of specific biological objects for researches and applications. In this study mangrove-derived bacteria were isolated and screened for antimicrobial producers. Also the strains with potential antibacterial activity were identified by 16S rRNA gene analysis. Results showed that 43 of 21 strains possessed antibacterial activity. The strain R10 showed inhibition against all four indicator bacteria and was identified as Bacillus pumilus with the highest sequence similarity of 100 to Bacillus pumilus NCTC10337 GenBank accession number . The strain R7 inhibited Serratia marcescens causing severe white spot disease in reef building coral Acropora palmata and was closely related to Bacillus toyonesis BCT 7112 GenBank accession number with 99 sequence similarity. The strain R3 was the strongest strain against Bacillus subtilis compared with other strains which revealed the same antimicrobial activity pattern. The strain R3 was affiliated with Bacillus marisflavi TF-11 GenBank accession number with 98 sequence similarity and may be considered to represent new species. Moreover the strain R9 inhibited only Gram negative tested strain and this strain was considered as a potential candidate for further search of antibiotics with spectrum of Gram negative bacteria. Keywords .