tailieunhanh - A voltammetric epithelial sodium channels immunosensor using screen-printed carbon electrode modified with reduced graphene oxide

This study aims to develop an electrochemical immunosensor for detecting ENaC using a screen-printed carbon electrode (SPCE), which was modified with reduced graphene oxide (RGO). | A voltammetric epithelial sodium channels immunosensor using screen-printed carbon electrode modified with reduced graphene oxide Current Chemistry Letters 9 2020 151 160 Contents lists available at GrowingScience Current Chemistry Letters homepage A voltammetric epithelial sodium channels immunosensor using screen-printed carbon electrode modified with reduced graphene oxide Yeni Wahyuni Hartatia Sonya Fatimah Yusupa Fitrilawatib Santhy Wyantutia Yulia Sofiatinc and Shabarni Gaffara a Department of Chemistry Faculty of Mathematics and Natural Sciences Universitas Padjadjaran Bandung Indonesia b Department of Physics Faculty of Mathematics and Natural Sciences Universitas Padjadjaran Bandung Indonesia c Department of Public Health Faculty of Medicine Universitas Padjadjaran Bandung Indonesia CHRONICLE ABSTRACT Article history Epithelial sodium channels ENaC is a protein that plays a role in maintaining sodium levels Received October 8 2019 and can be used as a biomarker of hypertension. This study aims to develop an electrochemical Received in revised form immunosensor for detecting ENaC using a screen-printed carbon electrode SPCE which was November 21 2019 modified with reduced graphene oxide RGO . The RGO improves the performance of Accepted February 18 2020 immunosensor because it contains -COOH groups that can form a covalent bond with the Available online terminal -NH2 of anti-ENaC. The result shows that the optimum concentration of anti-ENaC February 18 2020 was µg mL for 30 min incubation time. The various concentration of ENaC has a linear Keywords relationship with the decreasing of ferricyanide peak current signal with the range of Epithelial sodium channels ng mL and the limit of detection was ng mL. The level of ENaC concentration in urine ENaC samples can be used for the realization of a point-of-care device for early detection of Anti-ENaC hypertension. Hypertension Electrochemical immunosensor Reduced .