tailieunhanh - Identification and characterization of drought-responsive CC-type glutaredoxins from cassava cultivars reveals their involvement in ABA signalling

CC-type glutaredoxins (GRXs) are plant-specific glutaredoxin, play regulatory roles in response of biotic and abiotic stress. However, it is not clear whether the CC-type GRXs are involve in drought response in cassava (Manihot esculenta), an important tropical tuber root crop. | Identification and characterization of drought-responsive CC-type glutaredoxins from cassava cultivars reveals their involvement in ABA signalling .den color inherit .ttnd ol .ttnd ul .ttnd dl padding 0 0px 0 20px .ttnd hr margin 10px 0px .ttnd a href javascript void 0 .ttnd a href color inherit dtextscript p text-align left dtextscript img vertical-align middle Ruan et al. BMC Plant Biology 2018 18 329 lt br gt https s12870-018-1528-6 lt br gt lt br gt lt br gt lt br gt lt br gt RESEARCH ARTICLE Open Access lt br gt lt br gt Identification and characterization of lt br gt drought-responsive CC-type glutaredoxins lt br gt from cassava cultivars reveals their lt br gt involvement in ABA signalling lt br gt Meng-Bin Ruan1 5 Yi-Ling Yang2 Kai-Mian Li3 Xin Guo4 Bin Wang4 Xiao-Ling Yu1 5 and Ming Peng1 5 lt br gt lt br gt lt br gt Abstract lt br gt Background CC-type glutaredoxins GRXs are plant-specific glutaredoxin play regulatory roles in response of biotic and lt br gt abiotic stress. However it is not clear whether the CC-type GRXs are involve in drought response in cassava Manihot lt br gt esculenta an important tropical tuber root crop. lt br gt Results Herein genome-wide analysis identified 18 CC-type GRXs in the cassava genome of which six namely lt br gt MeGRXC3 C4 C7 C14 C15 and C18 were induced by drought stress in leaves of two cassava cultivars Argentina 7 Arg7 lt br gt and South China 124 SC124 . Exogenous abscisic acid ABA application induced the expression of all the six CC-type lt br gt GRXs in leaves of both Arg7 and SC124 plants. Overexpression of MeGRXC15 in Arabidopsis Col-0 increases tolerance of lt br gt ABA on the sealed agar plates but results in drought hypersensitivity in soil-grown plants. The results of microarray assays lt br gt show that MeGRXC15 overexpression affected the expression of a set of transcription factors which involve in stress lt br gt response ABA and JA ET signalling pathway. The results of protein .