tailieunhanh - The walnut transcription factor JrGRAS2 contributes to high temperature stress tolerance involving in Dof transcriptional regulation and HSP protein expression

GRAS transcription factor (TF) family is unique and numerous in higher plants with diverse functions that involving in plant growth and development processes, such as gibberellin (GA) signal transduction, root development, root nodule formation, and mycorrhiza formation. | Nội dung Text The walnut transcription factor JrGRAS2 contributes to high temperature stress tolerance involving in Dof transcriptional regulation and HSP protein expression .den color inherit .ttnd ol .ttnd ul .ttnd dl padding 0 0px 0 20px .ttnd hr margin 10px 0px .ttnd a href javascript void 0 .ttnd a href color inherit dtextscript p text-align left dtextscript img vertical-align middle Yang et al. BMC Plant Biology 2018 18 367 lt br gt https s12870-018-1568-y lt br gt lt br gt lt br gt lt br gt lt br gt RESEARCH ARTICLE Open Access lt br gt lt br gt The walnut transcription factor JrGRAS2 lt br gt contributes to high temperature stress lt br gt tolerance involving in Dof transcriptional lt br gt regulation and HSP protein expression lt br gt Guiyan Yang1 2 Xiangqian Gao1 2 Kaiheng Ma2 Dapei Li1 2 Caixia Jia2 Meizhi Zhai1 and Zhenggang Xu3 lt br gt lt br gt lt br gt Abstract lt br gt Background GRAS transcription factor TF family is unique and numerous in higher plants with diverse functions lt br gt that involving in plant growth and development processes such as gibberellin GA signal transduction root lt br gt development root nodule formation and mycorrhiza formation. Walnut tree is exposed to various environmental lt br gt stimulus that causing concern about its resistance mechanism. In order to understand the molecular mechanism of lt br gt walnut to adversity response a GRAS TF JrGRAS2 was cloned and characterized from Juglans regia in this study. lt br gt Results A 1500 bp promoter fragment of JrGRAS2 was identified from the genome of J. regia in which the lt br gt cis-elements were screened. This JrGRAS2 promoter displayed expression activity that was enhanced significantly by lt br gt high temperature HT stress. Yeast one-hybrid assay transient expression and chromatin immunoprecipitation lt br gt Chip -PCR analysis revealed that JrDof3 could specifically bind to the DOFCOREZM motif and share similar lt br gt expression patterns with .