tailieunhanh - RAD-seq reveals genetic structure of the F2-generation of natural willow hybrids (Salix L.) and a great potential for interspecific introgression

Hybridization of species with porous genomes can eventually lead to introgression via repeated backcrossing. The potential for introgression between species is reflected by the extent of segregation distortion in later generation hybrids. | Nội dung Text RAD-seq reveals genetic structure of the F2-generation of natural willow hybrids Salix L. and a great potential for interspecific introgression .den color inherit .ttnd ol .ttnd ul .ttnd dl padding 0 0px 0 20px .ttnd hr margin 10px 0px .ttnd a href javascript void 0 .ttnd a href color inherit dtextscript p text-align left dtextscript img vertical-align middle Gramlich et al. BMC Plant Biology 2018 18 317 lt br gt https s12870-018-1552-6 lt br gt lt br gt lt br gt lt br gt lt br gt RESEARCH ARTICLE Open Access lt br gt lt br gt RAD-seq reveals genetic structure of lt br gt the F2-generation of natural willow lt br gt hybrids Salix L. and a great potential lt br gt for interspecific introgression lt br gt Susanne Gramlich Natascha Dorothea Wagner and Elvira Hörandl lt br gt lt br gt lt br gt Abstract lt br gt Background Hybridization of species with porous genomes can eventually lead to introgression via repeated lt br gt backcrossing. The potential for introgression between species is reflected by the extent of segregation distortion in lt br gt later generation hybrids. Here we studied a population of hybrids between Salix purpurea and S. helvetica that has lt br gt emerged within the last 30 years on a glacier forefield in the European Alps due to secondary contact of the parental lt br gt species. We used 5758 biallelic SNPs produced by RAD sequencing with the aim to ascertain the predominance of lt br gt backcrosses F1 hybrid x parent or F2 hybrids F1 hybrid x F1 hybrid among hybrid offspring. Further the SNPs were lt br gt used to study segregation distortion in the second hybrid generation. lt br gt Results The analyses in STRUCTURE and NewHybrids revealed that the population consisted of parents and F1 hybrids lt br gt whereas hybrid offspring consisted mainly of backcrosses to either parental species but also some F2 hybrids. Although lt br gt there was a clear genetic differentiation between S. purpurea and S. helvetica FST .