tailieunhanh - Identification and characterization of GmMYB118 responses to drought and salt stress

Abiotic stress severely influences plant growth and development. MYB transcription factors (TFs), which compose one of the largest TF families, play an important role in abiotic stress responses. | Nội dung Text Identification and characterization of GmMYB118 responses to drought and salt stress .den color inherit .ttnd ol .ttnd ul .ttnd dl padding 0 0px 0 20px .ttnd hr margin 10px 0px .ttnd a href javascript void 0 .ttnd a href color inherit dtextscript p text-align left dtextscript img vertical-align middle Du et al. BMC Plant Biology 2018 18 320 lt br gt https s12870-018-1551-7 lt br gt lt br gt lt br gt lt br gt lt br gt RESEARCH ARTICLE Open Access lt br gt lt br gt Identification and characterization of lt br gt GmMYB118 responses to drought and salt lt br gt stress lt br gt Yong-Tao Du1 Meng-Jie Zhao1 Chang-Tao Wang2 Yuan Gao1 Yan-Xia Wang3 Yong-Wei Liu4 Ming Chen1 lt br gt Jun Chen1 Yong-Bin Zhou1 Zhao-Shi Xu1 and You-Zhi Ma1 lt br gt lt br gt lt br gt Abstract lt br gt Background Abiotic stress severely influences plant growth and development. MYB transcription factors TFs lt br gt which compose one of the largest TF families play an important role in abiotic stress responses. lt br gt Result We identified 139 soybean MYB-related genes these genes were divided into six groups based on their lt br gt conserved domain and were distributed among 20 chromosomes Chrs . Quantitative real-time PCR qRT-PCR lt br gt indicated that GmMYB118 highly responsive to drought salt and high temperature stress thus this gene was lt br gt selected for further analysis. Subcellular localization revealed that the GmMYB118 protein located in the nucleus. lt br gt Ectopic expression EX of GmMYB118 increased tolerance to drought and salt stress and regulated the expression lt br gt of several stress-associated genes in transgenic Arabidopsis plants. Similarly GmMYB118-overexpressing OE soybean lt br gt plants generated via Agrobacterium rhizogenes A. rhizogenes -mediated transformation of the hairy roots showed lt br gt improved drought and salt tolerance. Furthermore compared with the control CK plants the clustered regularly lt br gt interspaced short .

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