tailieunhanh - Maize male sterile 33 encodes a putative glycerol-3-phosphate acyltransferase that mediates anther cuticle formation and microspore development

The anther cuticle, which is primarily composed of lipid polymers, is crucial for pollen development and plays important roles in sexual reproduction in higher plants. However, the mechanism underlying the biosynthesis of lipid polymers in maize (Zea mays. L.) remains unclear. | Nội dung Text Maize male sterile 33 encodes a putative glycerol-3-phosphate acyltransferase that mediates anther cuticle formation and microspore development .den color inherit .ttnd ol .ttnd ul .ttnd dl padding 0 0px 0 20px .ttnd hr margin 10px 0px .ttnd a href javascript void 0 .ttnd a href color inherit dtextscript p text-align left dtextscript img vertical-align middle Zhang et al. BMC Plant Biology 2018 18 318 lt br gt https s12870-018-1543-7 lt br gt lt br gt lt br gt lt br gt lt br gt RESEARCH ARTICLE Open Access lt br gt lt br gt Maize male sterile 33 encodes a putative lt br gt glycerol-3-phosphate acyltransferase that lt br gt mediates anther cuticle formation and lt br gt microspore development lt br gt Lei Zhang1 Hongbing Luo2 Yue Zhao1 Xiaoyang Chen1 Yumin Huang1 Shuangshuang Yan3 Suxing Li1 lt br gt Meishan Liu1 Wei Huang1 Xiaolan Zhang3 and Weiwei Jin1 lt br gt lt br gt lt br gt Abstract lt br gt Background The anther cuticle which is primarily composed of lipid polymers is crucial for pollen development lt br gt and plays important roles in sexual reproduction in higher plants. However the mechanism underlying the lt br gt biosynthesis of lipid polymers in maize Zea mays. L. remains unclear. lt br gt Results Here we report that the maize male-sterile mutant shrinking anther 1 sa1 which is allelic to the classic lt br gt mutant male sterile 33 ms33 displays defective anther cuticle development and premature microspore lt br gt degradation. We isolated MS33 via map-based cloning. MS33 encodes a putative glycerol-3-phosphate lt br gt acyltransferase and is preferentially expressed in tapetal cells during anther development. Gas chromatography- lt br gt mass spectrometry revealed a substantial reduction in wax and cutin in ms33 anthers compared to wild type. lt br gt Accordingly RNA-sequencing analysis showed that many genes involved in wax and cutin biosynthesis are lt br gt differentially expressed in ms33 compared to wild type. lt br