tailieunhanh - Application of the CRISPR/Cas9 system for modification of flower color in Torenia fournieri

CRISPR/Cas9 technology is one of the most powerful and useful tools for genome editing in various living organisms. In higher plants, the system has been widely exploited not only for basic research, such as gene functional analysis, but also for applied research such as crop breeding. | Application of the CRISPR Cas9 system for modification of flower color in Torenia fournieri .den color inherit .ttnd ol .ttnd ul .ttnd dl padding 0 0px 0 20px .ttnd hr margin 10px 0px .ttnd a href javascript void 0 .ttnd a href color inherit dtextscript p text-align left dtextscript img vertical-align middle Nishihara et al. BMC Plant Biology 2018 18 331 lt br gt https s12870-018-1539-3 lt br gt lt br gt lt br gt lt br gt lt br gt RESEARCH ARTICLE Open Access lt br gt lt br gt Application of the CRISPR Cas9 system for lt br gt modification of flower color in Torenia lt br gt fournieri lt br gt Masahiro Nishihara1 Atsumi Higuchi1 Aiko Watanabe1 and Keisuke Tasaki1 2 lt br gt lt br gt lt br gt Abstract lt br gt Background CRISPR Cas9 technology is one of the most powerful and useful tools for genome editing in various lt br gt living organisms. In higher plants the system has been widely exploited not only for basic research such as gene lt br gt functional analysis but also for applied research such as crop breeding. Although the CRISPR Cas9 system has been lt br gt used to induce mutations in genes involved in various plant developmental processes few studies have been lt br gt performed to modify the color of ornamental flowers. We therefore attempted to use this system to modify flower lt br gt color in the model plant torenia Torenia fournieri L. . lt br gt Results We attempted to induce mutations in the torenia flavanone 3-hydroxylase F3H gene which encodes a lt br gt key enzyme involved in flavonoid biosynthesis. Application of the CRISPR Cas9 system successfully generated pale blue lt br gt almost white flowers at a high frequency ca. 80 of regenerated lines in transgenic torenia T0 plants. Sequence lt br gt analysis of PCR amplicons by Sanger and next-generation sequencing revealed the occurrence of mutations such as lt br gt base substitutions and insertions deletions in the F3H target sequence thus indicating that the obtained phenotype .