tailieunhanh - Expression of pcDNA-Interferon Lambda-3 (pcDNA-IFN3)

Type III Interferons also known as Interferon lambdas (IFN s/IFNLs) constitute a recently described IFN with antiviral activities by signaling through a unique receptor complex composed of IFN s receptor 1 (IFN R1) and interleukin-10 receptor 2 (IL10R2). It also found to have more of immune modulatory activities in addition to antiviral activities. In the present study, the construct pcDNA-bovine IFNλ3 was administered intramuscularly to mice. The mice were sacrificed on 7th and 14th day to collect the different organs/tissues. The tissues were used to isolate total RNA and following cDNA conversion. Then, the tissues were analyzed for the expression of IFN gene by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). The results had shown that there was expression of given IFNλ3 in organs: leg muscle, liver and spleen and also in blood. | 2019 8 9 2585-2589 EXCELLENT PUBLISHERS International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 8 Number 09 2019 Journal homepage http Original Research Article https Expression of pcDNA-Interferon Lambda-3 pcDNA-IFNÀ3 S. Barathiraja Lijo John N. Vijay P. A. V. Gangadhara and G. R. Reddy Department of Veterinary Biochemistry Rajiv Gandhi Institute of Veterinary Education and Research RIVER Kurumbapet Puducherry- 605 009 India Corresponding author ABSTRACT Keywords Bovine Interferon Lambda3 pcDNA- IFNX3 Mice RT- PCR Article Info Accepted 24 AUgust 2019 Available Online 10 September 2019 Type III Interferons also known as Interferon lambdas IFNXs IFNLs constitute a recently described IFN with antiviral activities by signaling through a unique receptor complex composed of IFNXs receptor 1 IFNẦR1 and interleukin-10 receptor 2 IL10R2 . It also found to have more of immune modulatory activities in addition to antiviral activities. In the present study the construct pcDNA-bovine IFNX3 was administered intramuscularly to mice. The mice were sacrificed on 7th and 14th day to collect the different organs tissues. The tissues were used to isolate total RNA and following cDNA conversion. Then the tissues were analyzed for the expression of IFNẦ gene by Reverse Transcriptase-Polymerase Chain Reaction RT-PCR . The results had shown that there was expression of given IFNX3 in organs leg muscle liver and spleen and also in blood. Introduction Type III Interferons IFNs also known as IFN Lambdas IFNXs IFNLs were described as members of a new cytokine family Kotenko et al. 2003 . IFNXs signals through a heterodimeric receptor IFNXR1 and IL-10R2 and plays significant role in antiviral activity and also involve in initiation of immune response. Unlike the ubiquitously expressed type I and II IFN receptor complexes type III IFNXR1 which is expressed predominantly in .