tailieunhanh - Occurrence of marek’s disease in commercial layer chicken

Layer chicken, Marek’s disease, PCR, Histopathology Eight dead commercial layer birds of 38 weeks age were examined to know the cause of death. The total flock size was 10,000 and birds were raised in cages from day one of age. The total mortality over a period of 13 weeks was per cent. The birds appeared weak and emaciated. At necropsy, liver, spleen, kidney revealed tumour nodules. The tumours were firm in consistency and smooth when cut. Histopathology of liver, spleen and kidney were corroborated the gross lesions. Marek’s disease (MD) was confirmed by conducting polymerase chain reaction (PCR). | 2019 8 9 2422-2426 EXCELLENT PUBLISHERS International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 8 Number 09 2019 Journal homepage http Original Research Article https Occurrence of Marek s Disease in Commercial Layer Chicken R. Ravikumar J. Selvaraj G. A. Balasubramaniam S. Balakrishnan T. Lurthu Reetha and R. Thangathurai Department of Veterinary Pathology Veterinary College and Research Institute Orathanadu- 614 625 Tamil Nadu India Corresponding author ABSTRACT Keywords Layer chicken Marek s disease PCR Histopathology Article Info Accepted 22 August 2019 Available Online 10 September 2019 Eight dead commercial layer birds of 38 weeks age were examined to know the cause of death. The total flock size was 10 000 and birds were raised in cages from day one of age. The total mortality over a period of 13 weeks was per cent. The birds appeared weak and emaciated. At necropsy liver spleen kidney revealed tumour nodules. The tumours were firm in consistency and smooth when cut. Histopathology of liver spleen and kidney were corroborated the gross lesions. Marek s disease MD was confirmed by conducting polymerase chain reaction PCR . Introduction Marek s Disease MD is a highly contagious oncogenic and neuropathic disease of chickens caused by herpes virus and spreads in the environment through feather follicle and infect the birds through inhalation and responsible for great economic losses to the poultry industry world wide Calnek and Adldinger 1971 . Sporadic outbreaks of MD have been reported recently throughout the world in vaccinated flocks including India Raja et al. 2009 Gopal et al. 2012 . The present paper describes the occurrence of Marek s disease in layer birds raised entirely in cages. Materials and Methods Eight dead commercial layer birds of 38 weeks of age were examined to know the cause of death with a case history of conjunctivitis off