tailieunhanh - Characterization of recombinant bean yellow mosaic virus belongs to group-IV infecting gladiolus

Gladiolus is an important vegetatively propagated ornamental plant cultivated in different parts of the world. The major constrains for production quality and quantity of flowers in gladiolus is due to infection of different viruses. During survey, the gladiolus plants (Ten samples) with mosaic symptoms were collected from different farmer fields in Bangalore rural district and were confirmed for Bean yellow mosaic virus (BYMV) infection by ELISA and PCR using specific primers. Further compete genome of BYMV that infects gladiolus was amplified cloned and sequenced. The analysis showed that the genome of BYMV shared maximum nucleotide identity with BYMV isolates belonging to the group IV infecting different crops in India, Japan, USA and Taiwan. Further recombination analysis showed that most part of the genome was derived from BYMV isolates from the phylogenic group of IV and I to emerge as a new variant of BYMV infecting gladiolus. The significance of these findings is discussed. | Characterization of recombinant bean yellow mosaic virus belongs to group-IV infecting gladiolus