tailieunhanh - Antimicrobial activity of the crude extract derived from Streptomyces spp. associated with sponges

The objective of the present investigation was to isolate and identify the antimicrobial activity showing actinomycetes associated with sponges. Sponges such as Acanthella klethra, Theonella cylendrica, Helicona sp., Auletta sp., and Callyspongia sp. were collected at Sangumal, in Rameswaram island, Ramanathapuram District, Tamil Nadu, India. Isolates of 22 actinomycetes were isolated from sponges. Isolates No. 16 showed high antibacterial and antifungal activity and the isolate was identified as Streptomyces sp. | Antimicrobial activity of the crude extract derived from Streptomyces spp. associated with sponges 2018 7 8 1187-1194 International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 7 Number 08 2018 Journal homepage http Original Research Article https Antimicrobial Activity of the Crude Extract Derived from Streptomyces spp. Associated with Sponges S. Parimala1 M. Kandhasamy and . Chinnasamy1 PG and Research Department of Botany Government Arts College Autonomous Karur 639 005 India Corresponding author ABSTRACT The objective of the present investigation was to isolate and identify the antimicrobial activity showing actinomycetes associated with sponges. Sponges such as Acanthella Keywords klethra Theonella cylendrica Helicona sp. Auletta sp. and Callyspongia sp. were Sponges collected at Sangumal in Rameswaram island Ramanathapuram District Tamil Nadu Actinomycetes India. Isolates of 22 actinomycetes were isolated from sponges. Isolates No. 16 showed Human pathogens high antibacterial and antifungal activity and the isolate was identified as Streptomyces sp. Crude extract Antibacterial and antifungal activity was determined by agar diffusion method and Antibacterial Minimum Inhibitory Concentration MIC of the extract was determined by two fold activity Antifungal dilution method using microstate plate. The study showed that fungal pathogens were more activity Minimum susceptible to the extract than bacteria. Among bacterial pathogens Escherichia coli was inhibitory more susceptible to the extract followed by P. aeruginosa Bacillus subtilis and concentration Staphylococcus aureus and their growth were inhibited at the concentration of 2 mg ml. Article Info Other bacterial pathogens were resistant to the crude extract derived from culture broth extract of Streptomyces sp. Fungal pathogens such as Candida albicans Zynomonas sp. Accepted Aspergillus niger .