tailieunhanh - Antimicrobial susceptibility and genetic characterization of extended spectrum beta-lactamase (ESBL)-producing nosocomial strains of escherichia coli isolated from a Brazilian teaching hospital

Escherichia coli are important pathogens that cause serious infections in hospitalized patients. This study aimed to investigate the occurrence of β-lactamase genes in twelve ESBL-producing E. coli isolated from patients in the Santa Casa de Misericordia de Sobral-CE, a teaching hospital in Northeastern Brazil, and analyze the antimicrobial susceptibility profile of these microorganisms. | Antimicrobial susceptibility and genetic characterization of extended spectrum beta-lactamase ESBL -producing nosocomial strains of escherichia coli isolated from a Brazilian teaching hospital 2018 7 8 422-434 International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 7 Number 08 2018 Journal homepage http Original Research Article https Antimicrobial Susceptibility and Genetic Characterization of Extended Spectrum Beta-Lactamase ESBL -Producing Nosocomial Strains of Escherichia coli Isolated from a Brazilian Teaching Hospital Rafaele Aragão dos Santos Vicente de Paulo Teixeira Pinto and Francisco Cesar Barroso Barbosa Microbiology Laboratory FAMED Federal University of Ceará Avenida Comandante Maurocélio Rocha Pontes 100 Sobral Ceará Brazil Corresponding author ABSTRACT Escherichia coli are important pathogens that cause serious infections in hospitalized patients. This study aimed to investigate the occurrence of β-lactamase genes in twelve ESBL-producing E. coli isolated from patients in the Santa Casa de Misericordia de Keywords Sobral-CE a teaching hospital in Northeastern Brazil and analyze the antimicrobial Antimicrobial activity bla susceptibility profile of these microorganisms. Genetic detection of the blaCTX-M blaSHV genes Escherichia coli and blaTEM genes was performed by PCR. All isolates were susceptible to ertapenem and ESBL α-pinene β- citronellol Ipomoea meropenem 11 92 were sensitive to amikacin 9 75 to colistin imipenem and carnea Ipomoea asarifolia tigecycline. Moreover 92 were resistant to ceftriaxone cefuroxime cefuroxime axetil Teaching hospital and 100 to ampicillin and ciprofloxacin. The blaCTX-M gene was detected in 9 75 Article Info isolates blaSHV in 6 50 and blaTEM in 5 . In 3 25 of the isolates were detected simultaneously genes encoding all three types of β-lactamase investigated. Accepted Additionally the .