tailieunhanh - Catalytic conditions of fucoidanase from vasticardium flavum
In this paper, we report on the characterizations of a fucoidanase from marine shell Vasticardium flavum, which degrades fucoidan from sea cucumbers Stichopus variegatus, Holothuria spinifera containing α-1→3 glycoside bonds. | Catalytic conditions of fucoidanase from vasticardium flavum Vietnam Journal of Science and Technology 57 (1) (2019) 28-37 doi: CATALYTIC CONDITIONS OF FUCOIDANASE FROM VASTICARDIUM FLAVUM Huynh Hoang Nhu Khanh*, Vo Thi Dieu Trang, Pham Duc Thinh, Pham Trung San Nhatrang Institute of Technology Research and Application (NITRA), VAST, 02 Hung Vuong, Nha Trang, Khanh Hoa * Email: hhnkhanh@ Received: 30 March 2018; Accepted for publication: 30 November 2018 Abstract. Fucoidanases are widely distributed in both marine microorganisms and marine invertebrates, however the data on the properties of this enzyme are scarce. In the present study, we isolated the fucoidanase from gastrointestinal tracts of the marine shell Vasticardium flavum and determined its enzymatic properties. The fucoidanase cleaved 1→3-α-L-fucan link of fucoidan extracted from sea cucumbers Stichopus variegatus, Holothuria spinifera, did not cleave fucoidans from F. evanescens and F. vesiculosus including rotational α-1→4 and α-1→3 glycoside chains. This enzyme did neither catalyze the hydrolysis of fucoidans from U. pinnatifida, S. mcclurei, which belongs to the galactofucan group. The fucoidanase showed the best activity at pH 3-4 and 24 hours of incubation. The enzyme activity was enhanced by Ca2+, Ba2+, Co2+ and Mg2+ cations, but it was inhibited by the Cu2+, Sn2+, Fe2+ and Al3+ cations. After incubation at 65 °C for 5 min, the enzyme activity was completely disappeared. Keywords: fucoidanase, Vasticardium flavum, fucoidan, enzyme. Classification numbers: , . 1. INTRODUCTION Fucoidans are a family of polysaccharides found in brown seaweeds and some other marine organisms. These polysaccharides exhibit a lot of biological activities, such as anticoagulant, antithrombotic, anticancer, anti-inflammatory and immunomodulatory. For these reasons, they are interesting to scientists around the .
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