tailieunhanh - Molecular detection and characterization of china aster (Callistephus chinensis L. Nees.) phyllody phytoplasma

China aster (Callistephus chinensis L. Nees.) is one of the important flower crop in India. It belongs to the family Asteraceae is native to China. Phyllody disease in China aster was first reported during 1986 from Bengaluru, India and known to be transmitted by Orosius albicinctus. The disease was characterized by chlorosis, upright growth, small leaf, short internode, stunting, profuse vegetative growth and phyllody (transformation of floral organs into leaf-like structures). Molecular detection was carried out through PCR assay by extracting the total DNA from phyllody infected aster leaf by using nested PCR phytoplasma specific universal primers R16F2n/R16FR2and results revealed that the amplification of phytoplasmal specific PCR product of kb fragment corresponding to the 16S rDNA. A 16S rDNA sequence comparison of aster phyllody phytoplasma with the 16S rDNA gene sequences of other phytoplasmas obtained from NCBI database. The BLAST analysis revealed that aster phyllody phytoplasma had 99 per cent sequence similarity with Tomato big bud TBB1 (), Alfalfa phytoplasma (Sudan) AP2 (), Pisumsativum phyllody () phytoplasma. Furthermore, the phylogenetic tree constructed by using the software MEGA showed that aster phyllody phytoplasma clustered with the Tomato big bud TBB1 () phytoplasma. The characterization of the phytoplasma through phylogenetic analysis of nucleotide sequence of 16S rDNA region showed that niger phyllody phytoplasma belong to 16S rII phytoplasmal group. | Molecular detection and characterization of china aster (Callistephus chinensis L. Nees.) phyllody phytoplasma

• Sinh học
• China aster
• Molecular and detection
• Phyllody phytoplasma
• Characterization of china aster
• 16S rII phytoplasmal group
• Callistephus chinensis
• Crossability studies in China aster
• China aster [Callistephus chinensis (L ) Nees]
• Crosses was determined
• Path analysis
• Path analysis in China aster
• China aster [Callistephus Chinensis (L) Nees]
• Characters influencing the flower yield
• Relative heterosis
• Relative heterosis in F1 hybrids
• F1 hybrids of China Aster
• Different plant growth regulators
• Levels on floral yield
• Economics of China Aster
• Trichoderma harzianum
• Growth parameters
• Pot condition
• China aster caused
• Bioagents against fusarium
• Spraying frequency
• China aster [Callistephus chinensis (L
• ) Nees
• Foliar application of gibberellic acid
• Concentrations and spraying frequencies
• Vegetative and floral attributes
• Container and deterioration
• Seed deterioration pattern
• China aster during storage
• Seed deterioration
• Phenotypic coefficients of variation
• Genotypic coefficients of variation
• Genetic advance as percentage of mean
• China aster varieties
• China aster [Callistephus chinensis (L ) Nees ]
• Callistephus hortensis
• Phosphorous on growth
• Growth of China aster
• Recorded in plants
• Commercial heterosis
• Economic traits
• Economic traits in China aster
• Exploitation of commercial heterosis
• Value addition
• Novel colours
• Tinting techniques in China aster
• Standardization of tinting techniques
• Bio fertilizer
• Integrated nutrient management
• Flower quality of China aster
• Phosphate solublizing bacteria etc
• Planting dates
• Loose flower production
• Planting dates in China aster
• Flower yield and vase life
• Vase life of China aster
• F1 hybrids
• Flower quality
• Quantitative traits
• Their parents for qualitative
• Evaluation of China Aster
• Parents for growth
• Descriptive statistics analysis cost
• Input use pattern
• China aster cultivation
• Chikkaballapura district of Karnataka
• Cost returns and input use pattern
• Heritability and genetic advance
• Genetic variability
• F2 segregating population
• Callistephus chinensis L
• Phenotypic correlation
• Path co efficient analysis
• F4 Population
• Genetic advance in F4
• Additive type of gene action
• Qualitative and quantitative characters
• Cut flower
• Loose flower
• Callistephus chinensis Nees
• Vegetative growth
• Flowering characters
• Varietal evaluation of China aster
• Sub tropical region of west Bengal
• Hand weeding
• Number of flowers
• Flower yielddes on floral
• Yield parameters
• Chrysanthemum (Perennial)
• Growth and yield
• Yield of different flower crops
• Different flower crops
• Influence of pinching on growth