tailieunhanh - Genetic fidelity evaluation of micropropagated plantlets of vanda hybrid ‘Dr.Anek’

Micropropagation is a highly sought after technique in the commercial production of orchid plants. This has the advantage of providing large number of plants in a short period of time. But a major constrain in the in vitro propagation technique is the somaclonal variation. It is important to produce true to type planting materials especially in case of the hybrids as they are more prone to variations. The true to type nature of the micropropagated plantlets can be confirmed by genetic fidelity analysis. ISSR markers were utilized to confirm the parental nature of the micropropagated plantlets of Vanda hybrid ‘’ so as to validate the micropropagation protocol developed at the centre for the hybrid. Mother plants and their respective clones were subjected to clonal fidelity studies using ISSR assay with five ISSR primers reported for Vanda hybrids. ISSR assay was performed to detect the polymorphism in amplification patterns in the region between two SSR’s using UBC808, UBC 811, UBC 826, UBC 835 and UBC 841. Further, the amplification profile of mother plants and micropropagated plantlets generated by each selected primers was examined for maximum number of amplicons, polymorphism and molecular weight of amplicons. Percentage of polymorphism generated by each primer was also worked out. In the present study, four mother plants namely M1, M4, M5 and M8 and five each of respective regenerants were analysed for genetic stability using five ISSR primers out of which two primers namely UBC 808 and UBC 835 showed polymorphism. The average polymorphism was observed to be only per cent. Monomorphic bands revealed the genetic stability of progenies inherited from their parents indicating the trueto-type nature of plantlets and thus validating the micropropagation protocol developed for the hybrid. | Genetic fidelity evaluation of micropropagated plantlets of vanda hybrid ‘’