tailieunhanh - Histological and immunohistochemical observations of supramammary lymph node in sheep and goat

Lymph node involved in activation and maturation of lymphocytes and stimulates antigenic response against invading foreign substances. Supramammary lymph nodes had high significance in mastitis and other udder infections in small ruminants. For the present study, six supramammary lymph nodes of Madras Red ewe and Boer local she-goat were collected from Chennai corporation slaughter house. Histological and immunohistochemical observations were carried out. In both the species, supramammary lymph node was composed of two parts viz., parenchyma and connective tissue stroma. The lymph node was covered by a fibroelastic capsule composed of collagen, elastic and reticular fibres. Trabeculae originated from the capsule and divided the lymph node into many lobules. Parenchyma composed of an outer cortex and inner medulla. Cortex region contained several lymphoid follicles, which was made by outer mantle zone and inner germinal centre. Statistically no significant difference was observed in micrometric parameters such as capsule thickness, trabeculae thickness, lymphoid follicle diameter, germinal centre diameter and number of follicles per field (50x) between ewe and she-goat. Immunohistochemical staining for CD3+ „T‟ lymphocytes revealed their localization was found to be in interfollicular region and less in germinal centre. Medullary region showed sparsely located „T‟ lymphocytes. | Histological and immunohistochemical observations of supramammary lymph node in sheep and goat