tailieunhanh - Differential DNA methylation under drought stress in maize

DNA methylation plays a vital roles in plant response to drought stress. Therefore, the present study was carried out to investigate differential cytosines methylation in leaves of maize under drought stress by using AMP method. Thirty days old maize seedlings were subjected to water deficit for nine days and the leaves of drought stressed plants and control plants were collected and stored at -20 until DNA extraction and subsequence DNA analysis. We isolated and sequenced 18 differentially methylated fragments. DMF 4, DMF 10, and DMF 13 were found to be homologous to lncRNA genes, DMF3 is similar to protein dehydrationinduced 19 homologue 3 and DMF 18 is homologous to nudix hydrolase 2 gene. The obtained results indicated that lncRNAs are consider as important regulators in response of maize plant to drought stress. | EXCELLENT PUBLISHERS International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 8 Number 08 2019 Journal homepage http Original Research Article https Differential DNA Methylation under Drought Stress in Maize Nehal Sallam1 Mounir Moussa1 Mohamed Yacout 1 and Ayman El-Seedy1 Department of Genetics Faculty of Agriculture Alexandria University Alexandria 21545 Egypt Corresponding author ABSTRACT Keywords DNA Methylation Drought Stress Maize DNA analysis Article Info Accepted 22 July 2019 Available Online 10 August 2019 DNA methylation plays a vital roles in plant response to drought stress. Therefore the present study was carried out to investigate differential cytosines methylation in leaves of maize under drought stress by using AMP method. Thirty days old maize seedlings were subjected to water deficit for nine days and the leaves of drought stressed plants and control plants were collected and stored at -20 until DNA extraction and subsequence DNA analysis. We isolated and sequenced 18 differentially methylated fragments. DMF 4 DMF 10 and DMF 13 were found to be homologous to lncRNA genes DMF3 is similar to protein dehydration-induced 19 homologue 3 and DMF 18 is homologous to nudix hydrolase 2 gene. The obtained results indicated that lncRNAs are consider as important regulators in response of maize plant to drought stress. Introduction Drought is one of the abiotic stresses that affect plant growth productivity and cause significant crop losses on annual basis. Therefore it is important to know how plants respond to drought stress by altering its biological activities. Plant faces abiotic stress by epigenetic changes that modify gene expression of the drought-responsive genes Setter et al. 2011 Boyer et al. 2012 Kakumanu et al. 2012 and Mao et al. 2015 . Epigenetic changes such as cytosine DNA methylation in plants play crucial roles in gene expression and silencing of transposon.

• Nông nghiệp
• DNA Methylation
• Drought Stress
• DNA analysis
• Drought stress in maize
• Differential DNA methylation
• BMC Cancer
• Breast cancer
• Methylation index
• Aberrant DNA methylation
• Genome Biology
• Non coding RNA
• ATP dependent chromatin remodeling
• RNA directed DNA methylation
• Several tested loci
• Genome wide DNA Methylation
• 5 MC quantification
• Methylation specific PCR
• Paediatric tuberculosis
• Genome wide global DNA methylation
• Germ Cell Tumor
• Epigenetic reprogramming
• Pluripotency related pathways
• Determining cellular identity
• Environmental responses
• Forcible methylation
• DNA epigenetic modifier enzymes
• BMC Genomics
• DNA integrity
• DNA fragmentation index
• Paternal genome
• Sperm DNA integrity
• BMC Bioinformatics
• Bisulfite sequencing
• DNA methylation patterns
• Methylation pattern
• Differential methylation analysis
• Downstream analysis
• Illumina Infinium methylation arrays
• Gene expression
• Non CpG methylation
• Prenatal homogenate cortex
• Cellular identity
• Methylation sensitive high resolution melting analysis
• Cystatin M
• Methylation specific PCR
• Phenotypic plasticity
• Genomic data
• Sociality through DNA Methylation
• Epigenetic control of sociality
• Prostate cancer
• Current diagnostic tools
• Non invasive diagnostic biomarker
• Allele specifc DNA methylation
• Alle lespecifc gene expression
• Eukaryotic organisms
• BMC Pulmonary Medicine
• Lung function
• Peripheral blood
• DNA methylation profiles
• Chronic obstructive pulmonary disease
• Endometrial stromal cells
• RNA expression
• Histone modification
• Bisulfite sequencing analysis
• Methylation co occurrence patterns
• Allele specific methylation
• Single locus modeling
• Precise prediction
• Logistic regression
• Differential methylation
• Sexual dimorphism
• Tissue specific methylation
• Cytosine methylation
• Spontaneous epiallele
• Epigenetic map
• Trans chromosomal methylation
• Methylation sensitive amplification polymorphism
• Tissue specificity
• Synergistic effect
• Nuclear DNA
• DNA methylation of alu repeats
• Complicated urinary tract infection
• Alu repeats
• Ethylation specific polymerase chain reaction
• Atopic asthma
• Gene expression profile
• DNA methylation profile
• DNA methylation/CpGs
• Gene ontology
• scientific reports
• thesis report
• medical report
• DNA methylation affects important developmental processes in both plants and animals
• The process of methylation of cytosines at C 5 is catalysed by DNA methyltransferases (MTases)
• which are highly conserved
• both struc turally and functionally
• in eukaryotes
• In this study
• we identified and characterized cytosine DNA MTase genes that are activated with the onset of reproductive development in rice
• 5 methylcytosine
• DNA damage
• Neuroimaging techniques
• Collect tumor tissue