tailieunhanh - Approaches for diagnosis of avian infectious bronchitis

Avian infectious bronchitis (IB) is one of the major economically important, worldwide distributed and highly contagious and acute upper‐ respiratory tract disease of poultry caused by avian infectious bronchitis virus (IBV) belonging to family Coronaviridae and affects both galliform and nongalliform birds. IBV demonstrates a wide range of tissues tropism, including the renal and reproductive systems. The clinical outcome of the infection in chickens depends on many variables such as the virus strain and type; sex and age of the chicken; immune status; co‐ infections; and environmental circumstances. Effective diagnostic tools are required to diagnose IB infections in field and to identify different serotypes and variants. Diagnosis of IB is done based on the clinical history, lesions, serological investigation, virus isolations and antigen detection. Current methods, such as reverse transcription polymerase chain reaction (RT-PCR), Restriction Fragment Length Polymorphism (RFLP) and sequencing offer highly sensitive, rapid, and accurate diagnostic results within the shortest possible time. This review discusses on approaches for diagnosis of avian infectious bronchitis infection. | 2019 8 9 539-553 EXCELLENT PUBLISHERS International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 8 Number 09 2019 Journal homepage http Review Article https Approaches for Diagnosis of Avian Infectious Bronchitis S. Shekhar1 . Sudhakar2 A. Kumar3 and S. Kumar4 1KrishiVigyan Kendra Koderma Jharkhand India 2 ICAR-National Institute of High Security Animal Diseases Bhopal India 3ICAR- Indian Veterinary Research Institute Bareilly India 4Bihar Veterinary College Patna India Corresponding author ABSTRACT Keywords Avian infectious bronchitis Coronavirus Poultry Diagnosis Serology Virus Isolation Molecular Methods Article Info Accepted 04 August 2019 Available Online 10 September 2019 Avian infectious bronchitis IB is one of the major economically important worldwide distributed and highly contagious and acute upper- respiratory tract disease of poultry caused by avian infectious bronchitis virus IBV belonging to family Coronaviridae and affects both galliform and nongalliform birds. IBV demonstrates a wide range of tissues tropism including the renal and reproductive systems. The clinical outcome of the infection in chickens depends on many variables such as the virus strain and type sex and age of the chicken immune status co- infections and environmental circumstances. Effective diagnostic tools are required to diagnose IB infections in field and to identify different serotypes and variants. Diagnosis of IB is done based on the clinical history lesions serological investigation virus isolations and antigen detection. Current methods such as reverse transcription polymerase chain reaction RT-PCR Restriction Fragment Length Polymorphism RFLP and sequencing offer highly sensitive rapid and accurate diagnostic results within the shortest possible time. This review discusses on approaches for diagnosis of avian infectious bronchitis infection. Introduction .

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