tailieunhanh - Molecular characterization of coat protein gene of blackgram yellow mosaic virus (BGYMV) from Karnataka, India

Yellow mosaic virus is the most destructive disease of urdbean causing 5-100 per cent yield loss. BGYMV belongs to genus Begomovirus of the family Geminiviridae transmitted by white fly (Bemisia tabaci Gennadius). Polymerase chain reaction of yellow mosaic virus infecting blackgram samples using MYMV-CP-F/MYMV-CP-R primers amplified the expected product of size 1000 bp from blackgram infected samples. Expected PCR products of size 1000 bp obtained were cloned, sequenced and assembled. | Molecular characterization of coat protein gene of blackgram yellow mosaic virus BGYMV from Karnataka India