tailieunhanh - Genetic divergence studies in subspecies of Oryza sativa L.

An experiment was carried out to study the divergence among twenty four rice genotypes (14 indica and 10 japonica lines) for 15 agro-morphological traits using D2 statistics and at molecular level using 114SSR markers. The cluster analysis using Mahalonobis’ D2 statistics classified the genotypes into five clusters. The cluster IV was the largest with seven indica and three tropical japonica types. The second largest cluster (V) accommodated four tropical japonica and two indica genotypes. The intra cluster distance ranged from to and it showed a gradual increase from cluster I to cluster V. Cluster III with four genotypes showed the highest inter cluster distance with cluster I (), followed by cluster II () and cluster IV (). Cluster II with AC 38479 and IR 64 showed the highest mean values for five traits viz., panicle length, number of primary branches panicle-1 , number of secondary branches panicle-1 , filled grains panicle-1 and single plant yield and the lowest mean values for hundred grain weight, kernel length, kernel breadth and kernel thickness which are desirable. The kernel traits viz., kernel L/B ratio (), 100 grain weight (), kernel thickness () and kernel breadth () alone contributed for of the total genetic divergence. In molecular diversity analysis, 69 SSR markers were detected to be polymorphic out of 114 markers. In total, 216 alleles were detected and the number of alleles amplified ranged between two to seven. The average number of alleles obtained was . The Polymorphism Information Content (PIC) which reflects the allelic diversity varied from (RM2) to (RM3317) and its average was . | 2019 8 7 834-845 EXCELLENT PUBLISHERS International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 8 Number 07 2019 Journal homepage http Original Research Article https Genetic Divergence Studies in Subspecies of Oryza sativa L. R. Sangeetha R. Saraswathi K. Amudha and G. Senthil Kumar Department of Rice Centre for Plant Breeding and Genetics Tamil Nadu Agricultural University Coimbatore - 03 Tamil Nadu India Corresponding author ABSTRACT Keywords Rice indica Tropical japonica D2 statistics Molecular diversity Article Info Accepted 07 JUne 2019 Available Online 10 July 2019 An experiment was carried out to study the divergence among twenty four rice genotypes 14 indica and 10 japonica lines for 15 agro-morphological traits using D2 statistics and at molecular level using 114SSR markers. The cluster analysis using Mahalonobis D2 statistics classified the genotypes into five clusters. The cluster IV was the largest with seven indica and three tropical japonica types. The second largest cluster V accommodated four tropical japonica and two indica genotypes. The intra cluster distance ranged from to and it showed a gradual increase from cluster I to cluster V. Cluster III with four genotypes showed the highest inter cluster distance with cluster I followed by cluster II and cluster IV . Cluster II with AC 38479 and IR 64 showed the highest mean values for five traits viz. panicle length number of primary branches panicle-1 number of secondary branches panicle-1 filled grains panicle-1 and single plant yield and the lowest mean values for hundred grain weight kernel length kernel breadth and kernel thickness which are desirable. The kernel traits viz. kernel L B ratio 100 grain weight kernel thickness and kernel breadth alone contributed for of the total genetic divergence. In molecular diversity .