tailieunhanh - Computational interaction analysis of cyprinus Carpio trypsin1 and Kunitz type soybean protease inhibitor

Common carp (Cyprinus carpio) is highly important culturable species in freshwater and cultured throughout the globe. Its farming require formulated feed with balance protein and energy. Most of the time fish meal is used as a protein source, but it is not acceptable from sustainability point of view. The replacement of fish meal by plant based protein source is advised. The limitation of plant based ingredients like soybean meal is the presence of anti-digestive factors in the form of several protease inhibitors. Kunitz type soybean protease inhibitor (SPI) is the most abundant protease inhibitor in soybean meal and it reduces the digestibility of feed. The data regarding molecular interaction and binding affinity of SPI with proteases is available in mammalian organism like pig but in fishes it has not been studied in detail. So the present study was conducted to gain insight into the protein-protein interaction and binding affinity of common carp trypsin (ccTrypsin1) and SPI using computational modelling. The sequence and 3D structure of ccTrypsin1 is highly similar to its mammalian counterpart pig. The protein-protein interaction analysis using knowledge based docking approach showed that the interaction was more or less similar like porcine trysin with SPI. The predicted binding affinity ( kcal/mol) and dissociation constant () showed strong interaction between the interactor governed by strong attractive forces like electrostatic forces. | Computational interaction analysis of cyprinus Carpio trypsin1 and Kunitz type soybean protease inhibitor

• Sinh học
• Cyprinus carpio
• Protease inhibitor
• Soybean meal
• Computational modelling
• Type soybean protease inhibitor
• Sublethal toxicity
• Physiological changes
• Fingerlings of Cyprinus carpio
• Sub lethal effects of dichlorvos
• Fermented water hyacinth
• Gut content
• Common carp (Cyprinus carpio)
• Equal amount of rice bran
• Organophosphate insecticide dichlorvos
• Acute toxicity
• Acute toxicity test
• Cadmium nitrate
• Cyprinus carpiro
• Lead nitrate
• 96 hours LC50
• Risk assessment of lead
• Cyprinus carpio in laboratory scale
• Trại nuôi trồng thủy sản
• Tích lũy kim loại
• Khảo sát nồng độ các kim loại
• Hàm lượng kim loại
• Kim loại tích lũy
• nghiên cứu khoa học
• báo cáo khoa học
• cá chép
• hàm lượng oxy
• Effect of cation concentrations
• Hormonal stimulation
• Common carp cyprinus carpio
• Effect of hormonal stimulation on sperm motility
• The institute of aquaculture in Nha Trang university
• Hàm lượng astaxanthin bổ sung vào thức ăn
• Tăng trưởng và màu sắc cá chép Koi (Cyprinus Carpio)
• Cá chép Koi
• Hàm lượng astaxanthin
• Amur common carp
• Physiological indices
• Female Amur common carp
• Physiological conditions
• Cyprinus carpio haematopterus
• Light emitting diode
• Cá chép Cyprinus carpio
• Nồng độ hormone IGF I mRNA
• Chỉ số tuyến sinh dục của cá chép
• Sinh sản của cá chép
• Common carp
• Polymerase chain reaction
• Cell culture assay
• Cyprinus carpio L
• Experimental infection with koi ranavirus
• Common carps
• Juvenile carps
• Cyprinus carpio var communis
• Dimethoate induced behavioural anomalies
• Organophosphate pesticide
• Sub lethal effects
• Bio chemical
• Sub lethal concentrations of dichlorvos
• Fingerling Cyprinus carpio
• Biochemical responses
• BMC Genomics
• Targeting analysis
• Gonad development
• Human drinking water source
• Aquatic ecological environment
• Genetic map
• QTL mapping
• Growth related traits
• Candidate gene
• BMC Veterinary Research
• Interferon regulatory factor 2
• Aeromonas hydrophila
• IFN response
• Immunoglobulin heavy chain
• BMC Genetics
• Linkage maps
• Multiple families
• Genetic improvement
• Selection response
• Heritability and common carp
• Estimated breeding values
• Luận án Tiến sĩ
• Chuyên ngành Ký sinh trùng
• Vi sinh vật học thú y
• Nghiên cứu dịch tễ học
• Ấu trùng sán lá
• Eisenia fetida
• Antibacterial activity
• Earthworm extract against pathogenic bacteria isolated
• Tilapia zilli
• Muscular tissue
• Staining bone technique