tailieunhanh - Phytochemical screening and antimicrobial effect of Ethanolic leaf extract of alstonia Boonei de wild (Apocynaceae) on some selected pathogenic micro-organisms

Antibiotics usage in animal production has been restricted by World Health Organization (WHO) due to their negative effects leading to the development of antibiotics resistant bacteria (superbugs) in animals and humans. The search for alternatives to antibiotic growth promoters has led to various researches in phytomedicine. An in-vitro experiment was therefore conducted to evaluate the antibacterial effect of ethanolic leaf extract of Alstonia boonei De Wild (Apocynaceae) on the following selected bacterial isolates: Escherichia coli, Salmonella typhi, Proteus mirabilis, Staphylococcus aureus and Pseudomonas aeruginosa. The antimicrobial effect was performed by agar well diffusion method. Preliminary phytochemical screening of the extract of Alstonia boonei indicated the presence of tannins, cardiac glycosides, flavonoids, saponnins, steroids and terpenoids while phlobatannins and anthraquinones were absent. The extract produced significant inhibitory effect on S. aureus and P. aeruginosa with inhibition zones of 19mm, 16mm, Minimum Inhibitory Concentration (MIC) of and respectively. E. coli, S. typhi and P. mirabilis were not sensitive to the extract. It was concluded that Staphylococcus aureus and Pseudomonas aeruginosa were highly sensitive to ethanolic leaf extract of Alstonia boonei. | Phytochemical screening and antimicrobial effect of Ethanolic leaf extract of alstonia Boonei de wild (Apocynaceae) on some selected pathogenic micro-organisms

• Hoá học
• Alstonia boonei
• Inhibition zones
• Selected pathogenic micro organisms
• Ethanolic leaf extract
• Phytochemical screening
• Hydnophytum formicarum Jack
• Botanical characteristic
• DNA barcodes
• Phytochemical study
• Preliminary screening of the phytochemical constituents
• Tacca Leontopetaloides
• Four geographical Locations
• Phytochemical analysis
• Secondary metabolites
• Fluorescence analysis
• Preliminary phytochemical
• Medicinal plants
• Azadirachta indica
• Melia azedarach
• Antimicrobial resistance
• Extracts of different parts
• Antibacterial assay
• Moringa oleifera lam
• Antimicrobial activity
• Vitex negundo leaf
• Stem extracts against bacterial
• Fungal pathogens
• Larvacidal activity
• Carica papaya
• Agave americana
• Piper nigrum
• Wrightia tinctoria
• Minimum inhibitory concentration
• Crude leaf extracts
• Erythrina variegate L
• Collection of plant sample
• Antimicrobial activity and phytochemical constituents
• Lemon grass oil extracts
• Oil extracts of neem leaves
• Solvent extraction method
• Hexane and ethanol
• Aloe vera
• A barbadensis miller
• TLC Bioautography
• Aloe vera (Aloe barbadensis Miller)
• Acacia catechu (L f) Willd
• Hydroethanolic leaf extract
• GCMS and phytoconstituents
• Acacia catechu
• Green tea
• Metal chelating ions
• Antioxidant activity
• Camellia sinensis
• Total phenolics content
• Phytochemical total phenolics
• Flavonoids contents of hibiscus rosa sinensis
• Quantify phenolcis
• Flavonoids contents in H Rosa sinensis
• Sybr green
• Plasmodium falciparum
• Crude extracts entandrophragma angolense
• Treatment of Malaria
• Cola lepidota
• Retention time
• Peak area
• Fatty acids
• Seed extract
• Pisonia alba
• Mukia maderaspatana
• Zone of inhibition
• APhytochemical screening
• Ocimum sanctum azadirachta indica
• Phyllanthus emblica
• Ocimum sanctum
• Journal of Chemistry
• Smilax china
• Salix alba
• Total antioxidant potential
• DPPH assay
• Antibacterial activity
• Escherichia coli
• Hibiscus rosa sinensis
• Staphylococcus aureus
• Total phenolic content
• Agar well diffusion method
• Salvia aegyptiaca
• Salvia aegyptiaca L
• Antimicrobial activity of various extracts
• Jatropha curcas
• Chlorophora excelsa
• Candida albicans
• Syzygium aromaticum
• Gallic acid
• Eugenyl acetate
• Syzygium aromaticum extract
• Essential oil
• Phlomis linearis
• Liquid chromatography mass spectromtetry (LC MS/MS)
• Gas chromatography mass spectrometry
• Chemical composition
• Biological activities
• Journal of Botany
• Kidney diseases
• Pharmacological activity
• Screening ethnobotanical
• Ethnomedicinaland phytochemical