tailieunhanh - Molecular characterization of coat protein gene of horse gram yellow mosaic Virus (HgYMV) from Southern India

Horse gram leaf samples showing yellow mosaic symptoms gave positive results with HgYMV specific primer pairs (HgYMV-CP-F/HgYMV-CP-R) and yielded amplicons of ~1000 bp whereas no PCR product was obtained from DNA extracted from healthy samples in PCR. The 1000 bp PCR products were cloned, sequenced, assembled and its length was determined as 885 bp including 111 bp pre-coat protein at 5’ end and 774 core coat protein. The length of the predicted amino acid sequence was found to be 257. Phylogenetic tree based on full length coat protein gene sequences of HgYMV with 23 other geminivirus sequences downloaded from NCBI Genbank formed three major clusters of MYMIV, HgYMV and MYMV. The present isolate formed unique cluster with HgYMV group that cause yellow mosaic disease symptoms in horsegram () and frenchbean (). Comparison of complete nucleotide sequence of coat protein gene of HgYMV indicated that coat protein gene of HgYMV-GKVK-Bangalore isolate had maximum homology with HgYMV-Tamil Nadu:HG [] (), followed by HgYMV-Srilanka:FB [] (). HgYMV-GKVK-Bangalore isolate had per cent identity and per cent identity with MYMV and MYMIV isolates, respectively. Comparison of predicted amino acid sequence of coat protein gene of HgYMV revealed that HgYMV-GKVK-Bangalore isolate shared maximum amino acid identity with HgYMV-Tamil Nadu:HG [] (), followed by HgYMV-Srilanka:FB [] (). HgYMV-GKVK-Bangalore isolate had per cent identity and per cent identity with MYMV and MYMIV isolates, respectively. From the above results, it is clear that HgYMV-GKVKBangalore is closely related to HgYMV isolates rather than MYMV and MYMIV. | Molecular characterization of coat protein gene of horse gram yellow mosaic Virus (HgYMV) from Southern India

• Sinh học
• Coat protein gene
• Yellow mosaic virus
• Phylogenetic analysis
• Sequence comparision
• Horse gram yellow mosaic Virus
• Coat protein
• Genomic RNA
• Zucchini yellow mosaic virus
• Sap transmission
• Gemini virus
• AV1 coat protein gene
• Chilli leaf curl virus
• Recombinant coat protein
• TA cloning
• His tag purification
• Cloning and expression
• Tomato leaf curl virus
• Banana bract mosaic virus
• Mysore poovan
• Molecular cloning and characterization
• Southern blot
• Expression vector
• Banana bunchy top viral coat protein
• Gene expression studies
• Molecular level
• Cucumber mosaic virus
• Infectious chlorosis
• Molecular characterization
• Complete coat protein gene sequence
• CMV infecting banana
• Blackgram yellow mosaic virus
• Nucleotide sequence identity
• Amino acid identity
• scientific reports
• thesis report
• medical report
• molecular Biology
• tumor cells
• Antimicrobial
• LAMP assay
• Ridge gourd
• Yellow mosaic disease
• Ridge gourd [Luffa acutangula (L) Roxb
• Loop mediated isothermal amplification assay