tailieunhanh - Isolation and characterization of a late embryogenesis abundant (LEA) protein coding gene (VrLEA2) from Mung Bean (Vigna radiata (L) Wilczek)

Mung bean is a widely consumed tropical food grain legume and is generally affected by different abiotic stresses. Late Embryogenic Abundant (LEA) proteins help the plants to withstand these stresses by its multi-functional role. In this study, a VrLEA2 gene isolated from mung bean using gene specific primers was sequenced and the in silico analysis of the gene sequence revealed the presence of eight ORF regions. ORF6region showed high similarity to Em like protein of Vigna radiata (L) Wilczek. The presence of conserved signature motif ‘GETVVPGGT’ revealed this protein to be hydrophilic and the presence of 20-mer aminoacid repeat ‘GGQTRKQQLGSEGYHEMGRK’ confirmed the protein to be belonging to Group 1 class of LEA protein families. Secondary structure prediction revealed it as coiled and disordered protein which specifies it has significant function of protecting biomolecules. The 3D structure of the protein was predicted using ROBETTA tool and was validated using PROCHECK, which found as better model based on several parameters. Further this gene can be validated in any expression system to understand the functional role played by this protein. | Isolation and characterization of a late embryogenesis abundant (LEA) protein coding gene (VrLEA2) from Mung Bean (Vigna radiata (L) Wilczek)