tailieunhanh - Isolation and characterization of a late embryogenesis abundant (LEA) protein coding gene (VrLEA2) from Mung Bean (Vigna radiata (L) Wilczek)

Mung bean is a widely consumed tropical food grain legume and is generally affected by different abiotic stresses. Late Embryogenic Abundant (LEA) proteins help the plants to withstand these stresses by its multi-functional role. In this study, a VrLEA2 gene isolated from mung bean using gene specific primers was sequenced and the in silico analysis of the gene sequence revealed the presence of eight ORF regions. ORF6region showed high similarity to Em like protein of Vigna radiata (L) Wilczek. The presence of conserved signature motif ‘GETVVPGGT’ revealed this protein to be hydrophilic and the presence of 20-mer aminoacid repeat ‘GGQTRKQQLGSEGYHEMGRK’ confirmed the protein to be belonging to Group 1 class of LEA protein families. Secondary structure prediction revealed it as coiled and disordered protein which specifies it has significant function of protecting biomolecules. The 3D structure of the protein was predicted using ROBETTA tool and was validated using PROCHECK, which found as better model based on several parameters. Further this gene can be validated in any expression system to understand the functional role played by this protein. | Isolation and characterization of a late embryogenesis abundant (LEA) protein coding gene (VrLEA2) from Mung Bean (Vigna radiata (L) Wilczek)

• Sinh học
• Mung bean
• VrLEA2 gene
• Sequence analysis
• Group 1 LEA proteins
• Structure validation
• Red soil
• Rhizobium and mung bean
• Grown in red soils of Mirzapur
• Boron application on yield
• Yield of mung bean
• Polyethylene glycol
• PEG treatments
• Germination rate
• Inhibited germination
• Mung bean seedlings
• Mung bean rhizosphere
• Characterization of rhizobacteria
• Multiple plant growth promoting traits
• Rhizobacterial isolates
• Yield components
• Nutritional traits
• Mung bean (Vigna radiata (L ) Wilczek)
• Nutritional traits in mung bean
• Heterosis for yield
• Date of sowing
• Irrigation regimes on growth
• Optimum date of sowing
• Districts etc
• Seed mycoflora of mung bean
• Prevalence of seed mycoflora
• Identification of seed mycoflora
• Performance of summer mung bean
• Summer mung bean
• Organic sources of nutrients
• Organic sources
• Bio fertilizers
• Nutrient content and uptake
• Uptake of buckwheat varieties
• Buckwheat varieties
• Residual effect on mung bean
• Journal of Science and Technology
• Effects of PH soaking solutions
• Hypoxia anaerobic treament
• Gaba accumulation in germinated Mung bean
• Vigna radiata L
• Carbonic anhydrase
• Nitrate reductase
• Stomatal conductance
• Mung bean Vigna radiata L
• 28 homobrassinolide
• Path analysis
• Yield and its components
• Correlation and path analysis studies
• Path analysis studies
• Effects of soaking on gamma aminobutyric aci
• Effects of germination conditions on gamma aminobutyric acid
• Total phenolic content in geminated mung bean
• Germination conditions on gamma aminobutyric acid
• Gamma – aminobutyric acid
• Pre harvest sprouting
• Pre harvest sprouting in mung bean
• Protein profiling against
• Chemical treatment
• Screening of genotypes
• Vegetative stage
• Reproductive stage
• Field condition in Bihar (India)
• Web blight
• Rhizoctonia solani
• Trichoderma harzianum
• Pseudomonas fluorescens
• Web blight disease of mung bean
• Invitro evaluation
• Evaluating the efficiency
• Efficiency of microbes
• Mung bean by different tests
• Improving crop yields
• Sustainable crop production
• Liquid formulations
• PSB inoculation
• PSB inoculation on growth
• Liquid formulations of PSB inoculation
• Vigna radiata
• Ipomoea aquatica
• Soil bacteria
• Water spinach
• Induced mutation
• Mungbean yellow mosaic virus
• Mutant population
• French bean
• Ascorbate peroxidase
• Guaiacol peroxidase
• Antioxidant enzymes
• Bean genotypes
• Lead and cadmium treatments
• Scientific reports
• scientific research
• biochemistry
• biochemical studies
• medical knowledge
• medical research
• Report medicine
• traditional medicine
• oxidation
• Crystal structure
• bacteria
• Soluble protein
• SPAD chlorophyll meter
• Double distilled water
• Phaseolus plants
• Effective microorganisms
• NPK fertilizers
• Organic amendments
• Lactic acid bacteria