tailieunhanh - Isolation and identification of PPFM bacterial isolates isolated from direct seeded rice growing areas of Hyderabad-karnataka, India

A total of 50 PPFM’s were isolated from rhizosphere soil and phyllosphere of direct seeded rice regions of Hyderabad-Karnataka. Samples of leaves and rhizosphere soils were collected from direct seeded rice grown in different location (Raichur district, Bellary district, Koppal district and Yadgir district). Thereafter, all the classical recommended methods used for identification of all the obtained isolates were carried out including, morphological, microscopical as well as biochemical characteristics. The results revealed that all the isolates were gram negative, rod shaped, motile. Colonies were pink, pale pink or dark pink in color due to pigmentation. Biochemical characteristics revealed that all the isolates including reference strain confirmed to be negative for Methyl red and VogesProskauer test while, positive for oxidase, urease, catalase activity, indole production and citrate utilization. None of the isolates could reduce nitrate to nitrite, whereas; the hydrolysis of casein and starch was not recorded in any of the isolates tested They can be cultivated on ammonium mineral salt (AMS) agar with methanol colonies were pink, pale pink or dark pink in color due to pigmentation and variations in colonial morphology. Based on the observed characteristics, the isolates obtained belong to the genus Methylobacterium. | Isolation and identification of PPFM bacterial isolates isolated from direct seeded rice growing areas of Hyderabad-karnataka, India