tailieunhanh - Identification of Trichoderma isolates from tobacco growing regions of west Godavari district, based on sequence analysis of its region of RDNA and morphological variations in the strains

The aim of this study was to determine the phylogenetic relationships of Trichoderma isolates obtained from NLS Tobacco region. Utilizing the sequence analysis of internal transcribed spacer-1 (ITS-1) region of the ribosomal DNA the rDNA of Five Trichoderma isolates was amplified by polymerase chain reaction (PCR) using universal primers (ITS-1 and ITS-4). PCR products were purified and these purified products were used to amplify the ITS-4 region of the five Trichoderma isolates. The amplified DNA was sequenced and aligned against ex-type strain sequences from TrichoBLAST and established Trichoderma taxonomy. Two isolates were positively identified as Trichoderma harzianum and three isolates were identified as Trichoderma aesperulum which were used as an outgroup in these analyses. The ITS-1 region sequences have been used as the reference’s sequence and this could be further used for future study involving the identification and taxonomy of Trichoderma Amplification of ITS-1 region of the rDNA. This method proved as a rapid and reliable technique for identifying Trichoderma strains successfully. | Identification of Trichoderma isolates from tobacco growing regions of west Godavari district, based on sequence analysis of its region of RDNA and morphological variations in the strains