tailieunhanh - Volatile composition, antioxidant property and antimicrobial activities against food-borne bacteria of Vietnamese thyme (Thymus vulgaris L.) essential oil

The essential oil (EO) obtained from the leaves of thyme (Thymus vulgaris L.) grown in Vietnam was found to contain thymol ( %), cymene ( %), and γ-terpinene ( %) as the main volatile components. The antimicrobial activities of this oil were screened against several food-borne bacteria and fungi species. Significant growth inhibition effects against food-borne bacteria Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Bacillus cereus (B. cereus), and Salmonella Typhimurium were observed using the standard disc diffusion method. Thyme EO exhibited the antibacterial effect against all the tested pathogenic strains. The inhibition zones were ± mm, ± mm, ± mm, 32 ± mm in diameter against B. cereus, E. coli, S. Typhimurium and S. aureus, respectively. The minimum inhibitory concentration (MIC) determined by the micro-dilution method in MHB liquid medium was µl/mL. The bactericidal concentrations (MBC) was µl/mL for three isolates from B. subtilis, E. coli, and S. aureus, while the MBC tested for S. Typhimurium was µl/mL. The antifungal properties of the thyme EO were also determined in this study against three important pathogenic fungi (Candida albicans, Rhizoctonia solani and Fusarium oxysporum) with the inhibition zones ranging approximately from ± to ± mm. On the other hands, the results also showed the antioxidant activity of Vietnamese thyme EO and suggested that thyme EO can be applied in food industries as natural flavoring preservatives/additives to control food spoilage and food-borne bacteria and fungi. | Volatile composition, antioxidant property and antimicrobial activities against food-borne bacteria of Vietnamese thyme (Thymus vulgaris L.) essential oil

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