tailieunhanh - Molecular Characterization of CTX-M15 Beta lactamase producing Escherichia coli isolates in intensive care units

The rapid emergence of multiresistant microbial pathogens is a serious threat to human health. Extended spectrum beta lactamase (ESBL)-producing Escherichia coli is a superbug causing worldwide outbreaks, CTX-M has become the most commonly detected ESBL genotype; these enzymes have become predominant worldwide, the most common type of CTX-M reported in various geographic regions is CTX-M15. A total of 80 Escherichia coli (E. coli) isolates were recovered from different clinical samples of intensive care units (ICUs) patients. These isolates were identified by Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS), in vitro antimicrobial susceptibility was investigated by VITEK 2 instrument, ESBL production was assessed phenotypically and ESBL genes (TEM, SHV and CTX-M15) confirmed genotypically by PCR and further confirmation for CTX-M15 by DNA sequencing. More than 50% of ESBL E. coli isolates were multidrug resistant; while carbapenams remains the most active compound among the studied isolates. Among ESBL E. coli, CTX-M-15 was 90% which was confirmed by sequencing. To our knowledge, this is the first report to study CTX-M-15 in E. coli isolates in Sharkia governorate. This study showed a high rate of ESBL E. coli with the widespread dissemination of CTX-M-15 which emphasize the need for employing an excellent management program in antibiotic therapy. | Molecular Characterization of CTX-M15 Beta lactamase producing Escherichia coli isolates in intensive care units

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• Molecular Characterization
• CTX M15 Beta lactamase producing
• Escherichia coli isolates
• Intensive care units
• Excellent management program
• SSR markers
• G hirsutum using SSR markers
• Molecular characterization of germplasm accessions
• Germplasm accessions
• Molecular and markers
• SSR marker based molecular characterization
• SSR marker based
• Genetic relationships
• Oyster mushroom
• Molecular characterization of oyster mushroom
• Subtropical regions of the world
• Macro fungus with a distinctive fruiting body
• Group A rotavirus
• G and P genotyping
• Group A rotavirus in children
• Molecular characterization of group A rotavirus
• Bumble bee
• North east Himalayas
• Molecular characterization of bumble bees species
• Bumble bees species
• Cotton (Gossypium hirsutum L )
• SSR makers
• Molecular characterization of heterotic groups
• Cotton through SSR markers
• Molecular characterization of RNA3
• CMV infecting tomato
• Classified into two subgroups
• Basis of CP sequence homology
• Zea mays
• SSR marker
• Molecular characterization of maize
• Simple sequence repeats marker
• Molecular characterization of fenugreek
• Genotypes using rapd markers
• Rapd markers
• Polymorphism exhibited
• Magnaporthe grisea
• Bacillus strains against M grisea
• Magnaporthe grisea and bio efficacy
• Morphological and molecular characterization
• Marssonina coronaria
• ITS regions
• Premature leaf fall
• Purple blotch
• Purple blotch disease of onion
• Alternaria isolates
• Molecular characterization of wheat
• Triticum sp
• Genotypes grown
• Humid south eastern plain zone
• Molecular marker
• Papaver somniferum L
• Opium poppy (Papaver somniferum L
• ISSR Based molecular characterization
• ISSR similarity matrix revealed
• Molecular detection
• Clinico seroepidemiology
• Molecular characterization of brucellosis
• Brucellosis in animals
• Brucella antibodies by RBPT
• Fusarium wilt
• Bitter gourd
• Fusarium wilt of bitter gourd
• Black spot
• Asperisporium caricae
• Incited byasperisporium caricae
• Detection and molecular characterization
• Scientific reports
• breast cancer
• medicine
• characterization
• molecular
• cytoplasm
• Citrus tristeza virus
• Western blot
• Biological indexing
• Double antibody sandwich
• Enzyme linked immunosorbent assay
• Reverse transcription
• Genetic variability
• Structure of olive
• Microsatellite markers
• Olea europaea L
• Anaplasma phagocytophilum
• Rhipicephalus sanguineus
• Dogs from Egypt
• Human granulocytic anaplasmosis
• MD simulation
• Silico characterization
• Molecular dynamics simulation
• Expression analysis
• Class I chitinases
• Aloe barbadensis
• Poultry meat
• Antibiotic Sensitivity
• MecA gene
• Poultry house environment
• Methicillin resistant staphylococcus aureus obtained
• Sheath blight
• Rhizoctonia solani
• Causing sheath blight in rice
• Tetranychus urticae
• METI acaricides
• Mt COI
• Coi based molecular characterization
• Genetic analysis
• Fenazaquin selected resistant strain
• Two spotted spider mite