tailieunhanh - Detection of Mycobacterium tuberculosis in dog of Assam

A total of 52 suspected dogs with symptoms of harsh, chronic non-productive coughing, fever and anorexia were examined for tuberculosis in the Teaching Veterinary Clinical Complex, Guwahati, Assam. SIT carried out by injecting of tuberculin (2TU, 5TU and 10TU) at either the skin of the thorax at the level of the costochrondral junction or the skin on the medial aspect of the hind limb in the thigh region and blood was collected aseptically for IFN-γ assay. Out of which the 2 dogs were found positive for TST and 3 dogs were positive for IFN-gamma assay. Moreover, X‐ray was carried out in 4 suspected dogs and only one dog had shown suspected lesion in the lung. Post-mortem was also carried out in 3 suspected tuberculosis cases brought to the Teaching Veterinary Clinical Complex (TVCC), Guwahati, Assam. An isolate of Mycobacterium was recovered from the suspected lesion test and confirmed as Mycobacterium tuberculosis by biochemical test and PCR assay. These parameters may be used for diagnosis of tuberculosis in dog. | 2019 8 5 1283-1288 EXCELLENT PUBLISHERS International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 8 Number 05 2019 Journal homepage http Original Research Article https Detection of Mycobacterium tuberculosis in Dog of Assam Acheenta G. Barua1 Koushik Kakoty1 Pranjal M. Nath1 and Bhaben Baishya2 1 Department of Veterinary Public Health Teaching Velerinary Clinical Complex College of Veterinary Science Assam Agricultural University Khanapara Guwahati-781022 India Corresponding author ABSTRACT Keywords Dog Mycobacterium tSt IFN-y assay Article Info Accepted 12April 2019 Available Online 10 May 2019 A total of 52 suspected dogs with symptoms of harsh chronic non-productive coughing fever and anorexia were examined for tuberculosis in the Teaching Veterinary Clinical Complex Guwahati Assam. SIT carried out by injecting of tuberculin 2TU 5TU and 10TU at either the skin of the thorax at the level of the costochrondral junction or the skin on the medial aspect of the hind limb in the thigh region and blood was collected aseptically for IFN-y assay. Out of which the 2 dogs were found positive for TST and 3 dogs were positive for IFN-gamma assay. Moreover X-ray was carried out in 4 suspected dogs and only one dog had shown suspected lesion in the lung. Post-mortem was also carried out in 3 suspected tuberculosis cases brought to the Teaching Veterinary Clinical Complex TVCC Guwahati Assam. An isolate of Mycobacterium was recovered from the suspected lesion test and confirmed as Mycobacterium tuberculosis by biochemical test and PCR assay. These parameters may be used for diagnosis of tuberculosis in dog. Introduction Natural infection by Mycobacterium tuberculosisis uncommonly isolated from cases of animal tuberculosis following close prolonged contact with infectious humans Michel et al. 2003 . Companion animals living in contact with TB patients are at .