tailieunhanh - Molecular characterization of wheat (Triticum sp.) genotypes grown in humid south eastern plain zone of Rajasthan

Wheat is qualitatively a vital source of macromolecule, energy and fiber for human nutrition since decades hence, used as a staple food grain for community and as well a major source of fodder for animal feeding. Assessment of genetic diversity using molecular markers is used for characterization of different genotypes with reliable and authentic results. RAPD is a PCR based molecular technique for identification of genetic variability in similar genotypes. It usually preferred for the initiation of this kind of work as this technique is simple, versatile and relatively inexpensive. 35 amplified bands were obtained using 6 RAPD primers, in which () were polymorphic and () was monomorphic. Total amplified bands varied in between 5 to 7 with an average of bands/primer. Average PIC value was with ranging from to . The lowest and the highest PIC value were recorded for primer OPA 14 and OPA 05, respectively. UPGMA cluster constructed from RAPD analysis clubbed the 10 wheat genotypes into three major clusters I, II and III and I was further divided in sub clusters IA and IB. The eager knowledge of genetic diversity is used to diagnose genetic programme and beneficial for future crop improvement. | Molecular characterization of wheat (Triticum sp.) genotypes grown in humid south eastern plain zone of Rajasthan