tailieunhanh - DNA sequence variation within STAT1 gene of Gaolao cattle

The current research was designed to study DNA sequence variation within Signal transducer and activator of trancription 1 (STAT1) gene and to find association of STAT1 gene variants (if any) with milk traits in Gaolao cattle. DNA was isolated from around 40 Gaolao cows from farmers herd and farm in breeding tract. The STAT1 gene regions of 314 bp, 850 bp and 154 bp fragments were amplified using STAT1G1, STAT1G2 and STAT1G4 primers respectively. | 2020 9 3 2883-2893 EXCELLENT PUBLISHERS International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 9 Number 3 2020 Journal homepage http Original Research Article https DNA Sequence Variation within STAT1 Gene of Gaolao Cattle P. M. Khandare1 D. S. Kale1 P. G. Koringa2 D. V. Patil1 and A. P. Dhok3 1 Department of Animal Genetics and Breeding Nagpur Veterinary College Maharashtra Animal and Fishery Sciences University MAFSU Nagpur India 2Department of Animal Biotechnology College of Veterinary Science and . Anand Agricultural University Anand India 3Department of Animal Nutrition Nagpur Veterinary College Maharashtra Animal and Fishery Sciences University MAFSU Nagpur India Corresponding author ABSTRACT Keywords Gaolao Cattle PCR-RFLP STAT1 SNP Sequencing Article Info Accepted 22 February 2020 Available Online 10 March 2020 The current research was designed to study DNA sequence variation within Signal transducer and activator of trancription 1 STAT1 gene and to find association of STAT1 gene variants if any with milk traits in Gaolao cattle. DNA was isolated from around 40 Gaolao cows from farmers herd and farm in breeding tract. The STAT1 gene regions of 314 bp 850 bp and 154 bp fragments were amplified using STAT1G1 STAT1G2 and STAT1G4 primers respectively. PCR-RFLP analysis of 314 bp 3 UTR fragment of STAT1 gene using PagI restriction enzyme revealed the CC genotype in Gaolao cattle indicating monomorphic status of the locus. The alignment analysis of 222 bp STAT1G1 sequence revealed SNP T-C at 221st position as polymorphic site. STAT1G2 primer amplified 783 bp sequence which revealed 03 polymorphic sites as SNP G-T at 724th position SNP A-G at 730th position and SNP C-G at 736th position. The polymorphic sites were identified in STAT1 gene sequence indicating sequence variation in the STAT1 gene regions in Gaolao Cattle. The identified polymorphic .