tailieunhanh - Biodiesel production by pichia occidentalis MH879824.1

A lipid producing isolate (LPI 65) was identified by a molecular genetics technique as Pichia occidentalis was investigated for its ability to produce biodiesel. The isolate was screened for its ability to accumulate lipids within the cells by culturing on nitrogen-limited medium (NLM) and using Sudan black B staining technique. Estimation of lipid content % was performed. The effect of different glucose concentrations in NLM on dry biomass and lipid weight were conducted and results showed that the best concentration of glucose was 70 g/L. The possibility of using different wastes such as sugar cane molasses, waste food oil and shortening, whey permeate, and waste motor oil as carbon sources for lipid production were determined, and the results showed that the best waste for lipid production was waste motor oil. Optimization of fermentation conditions such as effect of NLM composition, NLM pH, incubation temperature, and incubation period were also investigated and results of these tests showed that the best lipid accumulation when NLM5, at pH and incubated at 28oC for 120 h. Fatty acids profile analysis achieved that the lipids extracted from the isolate mainly contained the principal fatty acids (triacylglycerol’s, TAGs) which are similar to that of vegetable oils mainly oleic, linoleic and palmitic acid. | 2019 8 4 2975-2987 EXCELLENT PUBLISHERS International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 8 Number 04 2019 Journal homepage http Original Research Article https Biodiesel Production by Pichia occidentalis Laila Ramadan Yasser Fathy Abdelaliem and Salem Abdelfattah Mahfoz Department of Agricultural Microbiology Faculty of Agricultural Fayoum University Egypt Corresponding author ABSTRACT Keywords Honey Oleaginous yeasts Biodiesel lipid extraction Fatty acids analysis 26S rDNA gene Nitrogen-limited medium Article Info Accepted 20 March 2019 Available Online 10 April 2019 A lipid producing isolate LPI 65 was identified by a molecular genetics technique as Pichia occidentals was investigated for its ability to produce biodiesel. The isolate was screened for its ability to accumulate lipids within the cells by culturing on nitrogen-limited medium NLM and using Sudan black B staining technique. Estimation of lipid content was performed. The effect of different glucose concentrations in NLM on dry biomass and lipid weight were conducted and results showed that the best concentration of glucose was 70 g L. The possibility of using different wastes such as sugar cane molasses waste food oil and shortening whey permeate and waste motor oil as carbon sources for lipid production were determined and the results showed that the best waste for lipid production was waste motor oil. Optimization of fermentation conditions such as effect of NLM composition NLM pH incubation temperature and incubation period were also investigated and results of these tests showed that the best lipid accumulation when NLM5 at pH and incubated at 28oC for 120 h. Fatty acids profile analysis achieved that the lipids extracted from the isolate mainly contained the principal fatty acids triacylglycerol s TAGs which are similar to that of vegetable oils mainly