tailieunhanh - Psp68, a dead box helicase confers salinity tolerance in transgenic pigeon pea

Legumes are an important part of human diet account for about 27% of global primary crop production. Pigeon pea is the world’s sixth most important and second most important legume pulse crop of India after chickpea and mainly cultivated as rain fed crop. Its production is adversely affected due to salinity in arid and semi-arid regions of world. Salt stress reduces water potential, creates imbalance in ion concentration and causes toxicity. Helicases have been shown to play an important role in plants against salt stress. p68 which is a prototype member of DEAD-box helicase interacts with Ca2+ -CaM, thus regulating diverse signalling pathways against salt stress in plants. In the present study, we have developed transgenic pigeon pea plants with marker free gene Psp68 for salinity tolerance. Since regeneration is prerequisite for transgenic development and pigeon pea is considered to be recalcitrant, the transgenic pigeon pea plants containing Psp68 gene have been developed using the tissue culture independent transformation method (Patent Application No. 201811012099). | Psp68, a dead box helicase confers salinity tolerance in transgenic pigeon pea

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