tailieunhanh - Isolation and characterization of Rhizobium associated with root nodules of Dalbergia Sissoo

A study was conducted to isolate and characterize the rhizobia from the root nodule of Dalbergia sissoo from five different sites of Himachal Pradesh (Bilaspur, Solan, Hamirpur, Kangra and Una) and Uttrakhand (Rishikesh, Nainital, Haridwar, Dehradun and Udham Singh Nagar). A total of 79 bacterial isolates were isolated from root nodule of Dalbergia sissoo on YEMA media after 3 days of incubation at 37˚C by serial dilution method. Out of 79 isolates only 38 isolates were identified as rhizobia on the basis of authentication test (Congo red test, Bromothymol blue test and Plant infection test). Thirty eight strains were characterized on the basis of biochemical characterization. On the basis of morphological properties all the isolates were round and slimy white with raised elevation and smooth surface indicating rhizobia. In Gram’s reaction they were pink, rod in shape indicating gram negative bacteria. Only two rhizobial isolates DBD1 and DRT5 were selected on the basis of plant growth promoting traits (P-solubilization, Nitrogen fixation, Siderophore production, HCN production and Biocontrol activity). These two rhizobial isolates may be useful to increase the symbiotic nitrogen fixation in legume tree and can be used as potential biofertilizer owing to their plant growth promoting characters. | Isolation and characterization of Rhizobium associated with root nodules of Dalbergia Sissoo

• Sinh học
• Nitrogen fixation
• Isolation and characterization
• Rhizobium associated
• Root nodules
• Dalbergia Sissoo
• Rhizobial symbiosis
• Nod factor
• Utilization in nitrogen fixation
• Plant genetic control
• Plant genetic control of nodulation
• Carbohydrate polymers
• Residual nitrogen
• Cellulosic material
• Ideal cellulose
• Biological nitrogen fixation
• Soil fertility
• Nitrogen economy
• Environmental terms
• Review on the role
• Cicer arietinum L Mesorhizobium
• Plant dry matter
• Nitrogen and phosphorus content
• Chickpea (Cicer arietinum L )
• Leguminous plants
• Nitrogen status of soybean
• Vegetative growth
• Biochemical mechanisms
• Cicer arietinum
• Symbiotic nitrogen fixation
• Micronutrient deficiency
• Chickpea Cicer arietinum L
• Cicer arietinum L
• N2 fixation
• Antagonistic effect
• Biological control bacteria pseudomonas
• Inhibits free nitrogen fixing bacteria
• Nitrogen fixing bacteria in the rhizosphere
• Glomus mosseae
• Mycorrhizal fungus
• Arbuscular mycorrhizal
• Nitrogen fixing bacteria
• Identification of nitrogenfixing bacteria isolated
• Agricultural soil
• 16S rRNA sequence similarity
• BMC Genomics
• Vulcanococcus limneticus sp nov
• Nitrogenase genes
• Genomic island
• Horizontal gene transfer
• High P soil
• Phosphorus fixation
• P fertility Regime
• Crop in high p soil
• Supplemental enhancement of nitrogen
• BMC Plant Biology
• Endophytic bacteria
• Oxalotrophic bacteria
• Vertical transmission
• Cysteine protease
• Programmed cell death
• Fixing atmospheric nitrogen
• Tripartite symbiotic
• cowpea genotypes
• poverty alleviation
• BNF applications
• sustainable agriculture
• Plant Nutrition
• Macronutrients
• Nutrient Uptake
• Nutritional Quality
• Plant growth promoting bacteria
• Indole acetic acid
• Soil chemical properties
• Phosphate solubilisation
• Low temperature
• Rhizobium leguminosarum subsp
• ciceri strains
• Wld chickpea
• Allochromatium vinosum
• Purple sulfur bacteria
• Anoxygenic phototrophic bacteria
• Rnf genes in Allochromatium vinosum
• Transcriptional analysis
• Clostridium beijerinckii
• Northern blotting
• Nif cluster
• Transcriptome characterization
• 454 pyrosequencing
• Gene ontology
• Molecular marker
• Transcriptome analysis
• Large scale identification
• Rhizobium leguminosarum bv
• ciceri
• Salt stress
• Wild chickpea
• Medicago truncatula
• Nodulation specificity
• Bacterial species
• Acid production
• Biotin requirement
• Tuberose rhizoplane in raichur
• Glucose peptone broth
• Bacillus sp
• Pseudomonas sp
• Amylase production
• Identification of Bacillus species
• Potassium solubilisation