tailieunhanh - A simple and efficient genomic DNA extraction protocol for dried leaf of threatened species Commiphora Wightii (Arnott) Bhandari for genetic analysis of plant biological system

Commiphora wightii (Arnott) Bhandari contains secondary metabolites, polysaccharides and phenolic compounds. The presence of secondary metabolites, reduce the yield and quality of the DNA. In the present study an alternative protocol for genomic DNA extraction from dry plant leaves was developed that is acquiescent to PCR-based genomic studies. Existing protocols were lengthy, costly or not appropriate for genomic DNA extraction from dry leaves. This modified CTAB (3%) and PVP (Polyvinylpyrrolidone) protocol include the use of NaCl, ß-mercaptoethanol in the extraction as well as application of autoclaved sand for proper grinding of dried leaves and inclusion of RNase A treatment in the protocol to fasten the process. The extracted DNA using present optimized protocol was super in quality and quantity. It was also suitable for polymerase chain reaction with random decamer, inter simple sequence repeat and barcode primers. The developed protocol is rapid and cost efficient with high quality and sufficient quantity of DNA for downstream PCR-based genetic analysis. | A simple and efficient genomic DNA extraction protocol for dried leaf of threatened species Commiphora Wightii (Arnott) Bhandari for genetic analysis of plant biological system