tailieunhanh - In vitro inductions of gamma ray mutation for fusarium wilt resistance and characterization of mutants in regionally grown banana cultiva

Regionally grown banana cultivars are severely infected by several pathogens one such Fusarium oxysoporum f. sp. Cubense which causes significant crop losses in the fields. In this study fusarium wilt screening was carried out on gamma ray induced mutants and molecular characterization was done to assess the acquired resistance to Fusarium oxysoporum f. sp. cubense. From the screening we observed that gamma ray derived mutants are more sensitive in 25, 30 and 35 Gy. Whereas, several mutants obtained from 40 and 45 were resistant as comparable with untreated control. Similar analysis with molecular markers also revealed comparable results in screening. SSR primers showed resistance specific bands and were used for the testing variation among mutants, after confirming its reproducibility. Putative resistant, moderate resistant and susceptible mutants along with untreated control banana plants when amplified with Ma 3/2 and Ma 15 in Nanjanagudu Rasabale showed the presence of a major band at 150 bp and 500 bp and in Ma 1/17 and STMS 7 in Ney Poovan showed the presence 500 bp and 250 bp as major bands, this evidenced the occurrence of random mutations in genome. | In vitro inductions of gamma ray mutation for fusarium wilt resistance and characterization of mutants in regionally grown banana cultiva

• Sinh học
• Vitro inductions
• Gamma ray mutation
• Fusarium wilt resistance
• Characterization of mutants
• Regionally grown banana cultiva